In my diploma I am presenting oral hygiene, dental plaque formation and in association with that, keystone pathogen hypothesis for periodontitis development. The characteristics of bacterium Aggregatibacter actinomycetemcomitans, which is strongly connected to periodontal disease and its Cdt toxin are described. The purpose of the diploma, was to determine the effect of the synthesis of recombinant Cdt toxinand its variant on the growth of the producing bacteria. The aim was to implement a test to analyze the effect of recombinant CdtB toxin variants on the survival of the producing Escherichia coli. To accomplish this, we applied basic molecular-biology techniques. We showed that we can discriminate between the CdtB toxin according to the count of the colony forming, and that the toxin variant, but not the wild type CdtB toxin prevent growth of the Escherichia coli.
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