Nanosekundni elektroporator z diodnim odpiralnim stikalom in silicij karbidnim MOSFET-om

PIRC, EVA (author), Reberšek, Matej (mentor)

Elektroporacija oziroma elektropermeabilizacija je pojav, pri katerem se zaradi prisotnosti električnega polja poveča prepustnost celične membrane. Opisana metoda se uporablja na področju medicine, biologije in biotehnologije. Za uspešno izvajanje potrebujemo elektroporator in elektrode. Elektroporator je pulzni generator, ki generira visokonapetostne pulze specifičnih oblik, amplitud in trajanja. Generator določa tudi število pulzov in njihovo ponavljalno frekvenco. Prvi cilj magistrskega dela je izbrati najbolj primerno gonilno vezje in MOSFET stikalo za izdelavo nanosekundnega nekaj kilovoltnega pulznega generatorja oziroma elektroporatorja. Osredotočimo se na novo tehnologijo silicij karbidnih MOSFET-ov, za katero pokažemo, da je najbolj primerna za izvedbo nanosekundnega generatorja z diodnim odpiralnim stikalom. Pri generatorju pravokotnih pulzov zasledimo namreč problem pojava sekundarnega pulza. Skozi magistrsko nalogo se najprej srečamo z oceno lastnosti in primerjavo izbranega elementa z najbolj pogosto uporabljenimi močnostnimi stikali in nato zaključimo s končno izvedbo in opisom delovanja ter meritvami izdelanega nanosekundnega elektroporatorja. Naprava generira 3.8 kV pulze Gauss-ove oblike na 50 Ω bremenu, dolžine 10 ns. V okviru naloge smo podrobno opisali izzive, s katerimi smo se srečali pri izdelavi naprave, podali naše rešitve za opisane izzive in možnosti za nadaljnjo izboljšavo naprave.

• Language: Slovenian
• Keywords: elektroporator, nanosekundni pulzni generator, silicij karbidni močnostni MOSFET, diodno odpiralno stikalo
• Work type: Master's thesis/paper
• Organization: FE - Faculty of Electrical Engineering
• Year: 2015
• PID: 20.500.12556/RUL-72134
• Publication date in RUL: 04.09.2015

PIRC, EVA, 2015, Nanosekundni elektroporator z diodnim odpiralnim stikalom in silicij karbidnim MOSFET-om [online]. Master’s thesis. [Accessed 14 April 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=72134

Secondary language

• Language: English
• Title: Nanosecond electroporator with diode opening switch and silicon carbide MOSFET

Abstract

Electroporation or electropermebilization is a technique in which an electrical field is applied to cells in order to increase the permeability of the cell membrane. This procedure is being successfully used in biology, biotechnology and medicine. The application requires electroporator and electrodes. An electroporator is a high voltage pulse generator that generates pulses of specific shape, amplitude, duration, number and pulse repetition frequency. The first goal of my master’s thesis is to choose the most appropriate driving circuit and MOSFET switch for the purpose of designing few kilo volts nanosecond pulse generator or electroporator. We focused on the new technology, a silicon carbide MOSFET’s which we believe is the most suitable technology for the realization of diode opening switch nanosecond pulse generator. In testing we noticed that a square pulse generator was inappropriate because of double pulse phenomenon. Throughout the master’s thesis we evaluate and compare new silicon carbide MOSFET with most commonly used technologies. Further on we show our design which incorporates new technology and knowledge gained from earlier evaluation. In addition, we give an explanation of the workings and measurements of our nanosecond pulse generator. The device generates 3.8 kV pulses of Gaussian shape and 10 ns duration on 50 Ω load. Within the thesis we have described in detail the challenge we have encountered during the design of the generator, presented our solutions for this challenges and possibilities for improvement.

• Keywords: electroporator, nanosecond pulse generator, silicon carbide power MOSFET transistor, diode opening switch