From the tunnels of oak pinhole borer Platypus cylindrus Fabr. (Coleoptera, Platypodidae) and ambrosia beetle Xyleborus monographus Fabr. (Coleoptera, Scolytidae) in oak wood the different species of microorganisms in laboratory conditions were successively isolated. In the galleries of P. cylindrus the following fungi were found Raffaelea ambrosiae, Ophiostoma piceae, O. piliferum, Ophiostoma sp. (anamorph Hyalorhinocladie/la), Hormonema dematioides, Ascoidea sp., Pichia sp., Trichoderma sp., Penicillium sp., Coniophora sp. and bacteria. Fungi species grown in tunnels of X. monographus were Ophiostoma piceae, Ophiostoma sp., Scedosporium? sp., Pichia sp., Trichoderma sp., Penicillium sp., Coniophora sp. and bacteria. Different species of fungi and their successive appearance in the galleries determine their role and importance tor the development of ambrosia beetles.
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