Enumration, isolation, and identification of bifidobacteria from dairy products

Vlková, Eva (author), Rada, Vojtěch (author), Trojanová, Iva (author)

Six dairy products were tested. Bifidobacteria were enumerated and isolated using TPY agar modified by the addition of mupirocin (100 mg l-1). Isolates were identified to the genus level by the detection of fructose-6-phosphate phosphoketolase (F6PPK) and by the FISH method. Bifidobacteria were characterised using API 50 CHL and API ID 32 A Rapid tests. In addition, grow at 46 °C was tested. Subsequently, all strains were identified to the species level using computer program Bacter. The identification was also carried out by PCR method using genus- and species-specific primers.

• Language: English
• Keywords: milk products, dairy products, microbiology, bifidobacteria, enumeration, identification, isolation
• Work type: Not categorized
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publisher: Biotehniška fakulteta
• Year: 2004
• Number of pages: Str. 31-36
• Numbering: Letn. 84, št. 1
• PID: 20.500.12556/RUL-61563
• UDC: 637.1
• ISSN on article: 1581-9175
• COBISS.SI-ID: 1677448
• Publication date in RUL: 10.07.2015

Record is a part of a journal

• Title: Acta agriculturae Slovenica
• Shortened title: Acta agric. Slov.
• Publisher: Biotehniška fakulteta
• ISSN: 1581-9175
• COBISS.SI-ID: 213840640

Secondary language

• Language: Slovenian
• Title: Štetje, izolacija in identifikacija bifidobakterij v mlečnih izdelkih

Abstract

Testirali smo šest mlečnih izdelkov. Štetje bifidobakterij in izolacijo smo izvedli na TPY agarju, ki smo mu dodali muriprocin (100 mg l-1). Izolate smo identificirali na ravni rodu z detekcijo fruktoza-6-fosfat fosfoketolaze (F6PPK) in z metodo FISH. Bifidobakterije smo opisali s hitrima testoma API 50CHL in API ID 32. Preverili smo tudi rast pri 46 °C. Nato smo vse isolate identificirali na ravni vrst z računalniškim programom Bacter. Dodatno smo izvedli identifikacijo z metodo PCR ob uporabi za posamezen rod, oziroma vrsto specifičnih začetnih oligonukleotidov.

• Keywords: mlečni izdelki, mikrobiologija, bifidobakterije, štetje, identifikacija, izolacija