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The microbiological quality of raw milk after introducing the two day's milk collecting system
ID Godič Torkar, Karmen (Author), ID Golc-Teger, Slavica (Author)

URLURL - Presentation file, Visit http://www.dlib.si/details/URN:NBN:SI:doc-52RUOPN7 This link opens in a new window

Abstract
This study was carried out to investigate the quality of raw milk after the decision of the dairies to collect the milk every two days and not daily as it has been performed till the year 2005. In wider central Slovenian production area we collected in winter and summer season 203 raw milk samples obtained from farm bulk milk tanks, collecting points and transportation tanks at the entrance to the dairy was studied. The total bacterial count, the number of coliforms, psychrotrophic micro-organisms, coagulase-positive staphylococci, yeasts and moulds were analysed using standard methods. The presence of mould species and production of aflatoxins were studied as well. The total bacterial count was higher than 100 000 cfu/ml in 48 (23.6%) out of all tested samples. Its mean value in all milk samples was 4.5 log10 cfu/ml . The mean numbers of coliform bacteria, psychrotrophic micro-organisms, yeasts and moulds together, and coagulase-positive staphzlococci were 2.1 log10 cfu/ml, 3.7 log10 cfu/ml, 2.3 log10 cfu/ml and 1.97 log10 cfu/ml, respectively. The yeasts were present in 95.0% of raw milk samples with the mean concentration of 1.7 log10 cfu/ml. Moulds were found in 63.3% of raw milk samples, their mean concentration was 0.6 log10 cfu/ml. Isolated mould strains belonged to genera Geotrichum (51.5%), Aspergillus (33.8%), Mucor (5.9%), Fusarium (2.9%) and Penicillium (2.9%). None of the isolated Aspergillus strains with typical growth on AFPA medium produced aflatoxin M1 on YES or YZGC medium supplemented with Methyl-ß-cyclodextrin.

Language:English
Keywords:raw milk, microbiological quality, total bacterial count, moulds, aflatoxin M1
Work type:Not categorized
Typology:1.01 - Original Scientific Article
Organization:BF - Biotechnical Faculty
Publisher:Biotehniška fakulteta
Year:2008
Number of pages:Str. 61-74
Numbering:Letn. 92, št. 1
PID:20.500.12556/RUL-57982 This link opens in a new window
UDC:637.1
ISSN on article:1581-9175
COBISS.SI-ID:2409096 This link opens in a new window
Publication date in RUL:10.07.2015
Views:1798
Downloads:290
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Record is a part of a journal

Title:Acta agriculturae Slovenica
Shortened title:Acta agric. Slov.
Publisher:Biotehniška fakulteta
ISSN:1581-9175
COBISS.SI-ID:213840640 This link opens in a new window

Secondary language

Language:Slovenian
Title:Mikrobiološka kakovost surovega mleka po uvedbi sistema dvodnevnega zbiranja
Abstract:
Preučevali smo mikrobiološko kakovost odkupljenega surovega mleka po odločitvi mlekarn, da bodo mleko zbirale vsake dva dni in ne več dnevno, kot so to izvajale do konca leta 2005. V osrednjem proizvodnem področju Slovenije smo v zimskem in letnem obdobju odvzeli 203 vzorcev mleka iz hladilnih bazenov posameznih proizvajalcev, zbiralnic ter transportnih cistern na sprejemu mlekarne. S standardnimi mikrobiološkimi metodami smo ugotavljali skupno število aerobnih mezofilnih mikroorganizmov, koliformnih in psihrotrofnih mikroorganizmov, število kvasovk in plesni ter koagulaza-pozitivnih stafilokokov. Ugotavljali smo tudi prisotnost posameznih vrst plesni in njihovo sposobnost tvorbe aflatoksinov. Skupno število mikroorganizmov je presegalo 100.000 ke/ml v 48 (23,6 %) od vseh preiskanih vzorcev. Njihovo povprečno število v vseh vzorcih je bilo 4,5 log10 ke/ml. Povprečno število koliformnih mikroorganizmov je znašalo 2,1 log10 ke/ml, število psihrotrofnih mikroorganizmov 3,7 log10 ke/ml, skupno število kvasovk in plesni 2,3 log10 ke/ml in število koagulaza-pozitivnih stafilokokov 1,97 log10 ke/ml. Kvasovke s povprečno koncentracijo 1,7 log10 ke/ml smo ugotovili v 95 % vzorcev, plesni s povprečno koncentracijo 0,6 log10 ke/ml pa v 63,3 % vzorcev. Najpogosteje smo izolirali plesni iz rodov Geotrichum (51,5%), Aspergillus (33,8 %), Mucor (5,9%), Fusarium (2,9%) in Penicillium (2,9%). Nobeden izmed izoliranih sevov iz rodu Aspergillus, ki je kazal značilno rast na gojišču AFPA, ni na gojiščih YES in YGC z metil-ß-ciklodekstrinom tvoril aflatoksina M1.

Keywords:surovo mleko, mikrobiološka kakovost, skupno število mikroorganizmov, plesni, aflatoksin M1

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