Polymorphism analysis of the promoter of cow lactoferrin gene with PCR-RFLP and its correlation with subclinical mastitis

Zhao, Chang-hong (author), He, Gao-ming (author), Wang, Yan-liang (author), Zhang, Zhao-xia (author)

CMT was used to detect the incidence of mastitis. One hundred twenty cows were selected and assigned into 2 groups, 60 animals in each group: control group (healthy cows), experimental group (cows with subclinical mastitis) and the relationship between cow's subclinical mastitis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the promoter of cow lactoferrin gene was explored. The results showed that polymorphism existed in the promoter of cow lactoferrin gene, which suggested that this polymorphism could be associated with mastitis susceptibility.

• Language: English
• Keywords: cattle, dairy cows, diseases, subclinical mastitis, molecular genetics, lactoferrin gene, PCR-RFLP
• Work type: Not categorized
• Typology: 1.03 - Short Scientific Article
• Organization: BF - Biotechnical Faculty
• Publisher: Biotehniška fakulteta
• Year: 2008
• Number of pages: Str. 185-187
• Numbering: Letn. 92, št. 2
• PID: 20.500.12556/RUL-57809
• UDC: 636.2
• ISSN on article: 1581-9175
• COBISS.SI-ID: 2415496
• Publication date in RUL: 10.07.2015

Record is a part of a journal

• Title: Acta agriculturae Slovenica
• Shortened title: Acta agric. Slov.
• Publisher: Biotehniška fakulteta
• ISSN: 1581-9175
• COBISS.SI-ID: 213840640

Secondary language

• Language: Slovenian
• Title: PCR-RFLP analiza polimorfizmov v promotorju laktoferinskega gena pri govedu in povezava s subkliničnim mastitisom

Abstract

Mastitis smo ugotavljali s na osnovi kliničnih znakov. Sto dvajset krav smo razdelili v dve skupini, 60 živali je bilo v kontrolni skupini (zdrave živali), drugih 60 pa v poskusni skupini (živali s subkliničnim mastitisom) in ugotavljali povezavo med subkliničnim mastitisom in polimorfizmom restrikcijskih fragmentov (PCR-RFLP) promotorske regije gena za laktoferin. Rezultati kažejo, da bi bil polimorfizem v promotorski regiji govejega laktoferinskega gena lahko povezan z dovzetnostjo za mastitis.

• Keywords: govedo, krave, molznice, bolezni, subklinični mastitis, molekularna genetika, geni, lactoferin, PCR-RFLP