Examples of high performance liquid chromatography (HPLC) application in marine ecology studies in the northern Adriatic

Flander-Putrle, Vesna (author)

Photosynthetic pigments have proved to be useful biomarkers of the abundance, composition and physiological status of the phytoplankton biomass in the marine environment. Using HPLC pigment analysis, we determined phytoplankton community structure in three different marine environments: in the area of a fish farm, in the area of sewage outlets, and in the mucilaginous aggregates. At the reference site we observed seasonal changes with prevalence of fucoxanthin-containing phytoplankton (i.e. diatoms) during winter/spring and autumn. In the fish farm area the concentration of chlorophyll a degradation products was higher, whereas in the locally enriched environment of sewage outlets we observed only small changes in taxonomic composition and phytoplankton biomass. The impact of season is more expressed than the impact of sewage discharge. With the use of HPLC pigment analysis we determined the development of phytoplankton community in different stages of mucilage aggregates. Phytoplankton biomass was composed primarily of diatoms, and as the aggregates aged, diatoms increased in the relative biomass. Our examples have proven the usefulness and suitability of HPLC pigment analysis in marine ecology studies.

• Language: English
• Keywords: liquid chromatography, Adriatic Sea, ecological status, HPLC, High performance liquid chromatography, pigments, Adriatic Sea, northern Adriatic, mucilage, fish farm, sewage outlets
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Year: 2010
• Number of pages: Str. 6-23
• Numbering: Vol. 12, št. 1
• PID: 20.500.12556/RUL-56713
• UDC: 574
• ISSN on article: 1580-0814
• COBISS.SI-ID: 2168399

Copyright

Licenca CC BY 4.0 Mednarodna navedena na pristajalni strani članka https://journals.uni-lj.si/NaturaSloveniae/article/view/16841 (7. 8. 2024)

• Publication date in RUL: 10.07.2015

Record is a part of a journal

• Title: Natura Sloveniae. revija za terensko biologijo
• Publisher: Zveza za tehnično kulturo Slovenije, Biotehniška fakulteta Univerze, Nacionalni inštitut za biologijo
• ISSN: 1580-0814
• COBISS.SI-ID: 102784768

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Title: Primeri uporabe tekočinske kromatografije visoke ločljivosti (HPLC) pri študijah morske ekologije severnega Jadrana

Abstract

Fotosintezna barvila so se izkazala kot dobri kazalci abundance, sestave in fiziološkega stanja fitoplanktonske biomase v morskem okolju. Z uporabo HPLC-analize barvil smo določili strukturo fitoplanktonske združbe v treh različnih morskih okoljih: na območju ribogojnic, na območju komunalnih podvodnih izpustov in v sluzastih makroagregatih. Na referenčni postaji smo izmerili sezonske spremembe fitoplanktonske združbe s prevlado fukoksantin-vsebujočega fitoplanktona (t.j. diatomej) v zimsko-spomladanskem in jesenskem obdobju. Na območju ribogojnic smo izmerili višje koncentracije razgradnih produktov klorofila a, na lokalno obogatenem območju komunalnih izpustov pa smo opazili le manjše spremembe v taksonomski sestavi in biomasi fitoplanktona. Vpliv sezone je bolj izražen kakor vpliv podvodnih komunalnih izpustov. Z uporabo HPLC-analize barvil smo določili razvoj fitoplanktonske združbe v posameznih razvojnih stadijih sluzastih makroagregatov. V fitoplanktonski združbi sluzastih makroagregatov so prevladovale diatomeje in njihov delež k relativni biomasi je s starostjo agregatov še naraščal. Navedeni primeri kažejo na uporabnost in primernost HPLC-analize barvil v študijah morske ekologije.

• Keywords: morje, pigmenti, fitoplankton, tekočinska kromatografija, ekološki status, sluzenje morja, onesnaževanje morja, obalno morje, HPLC, severni Jadran, Jadransko morje