Using different growth medium greatly improves distinction of Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans strains by the cellular fatty acids and aldehydes profiles

Zorec, Maša (author), Vodovnik, Maša (author), Marinšek-Logar, Romana (author)

A total of 11 ruminal strains currently assigned to Butyrivibrio fibrisolvens and Pseudobutyrivibrio xylanivorans were cultivated in two different media, rumen fluid containing M2 and short-chain fatty acid (SCFA ) containing M330, and their cellular fatty acid methyl esters (FA ME) and dimethylacetals (DMA) were analyzed using gas chromatography. A comparison of the FA ME/DMA compositions revealed that the difference in SCFA contents in the growth medium induced a pronounced quantitative effect on the cellular branched-chainfatty acid and aldehydes proportions only in the P. xylanivorans strains. This study shows that FA ME/DMA analysis is a powerful chemotaxonomic tool in the group of phenotypically similar rumen butyrivibria especially when the influence of the growth medium is evaluated.

• Language: English
• Keywords: microbiology, anaerobic bacteria, rumen, Butyrivibrio, Pseudobutyrivibrio, FAME, DMA
• Work type: Not categorized
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Year: 2011
• Number of pages: Str. 15-19
• Numbering: Letn. 98, št. 1
• PID: 20.500.12556/RUL-376
• UDC: 579
• ISSN on article: 1581-9175
• COBISS.SI-ID: 2899080
• Publication date in RUL: 11.07.2014

Record is a part of a journal

• Title: Acta agriculturae Slovenica
• Shortened title: Acta agric. Slov.
• Publisher: Biotehniška fakulteta
• ISSN: 1581-9175
• COBISS.SI-ID: 213840640

Secondary language

• Language: Slovenian

Abstract

Skupno smo gojili 11 vampnih sevov iz vrst Butyrivibrio fibrisolvens in Pseudobutyrivibrio xylanivorans v dveh različnih gojiščih: v M2 z vampnim sokom in v M330 z mešanico kratkoverižnih maščobnih kislin (SCFA ). S plinsko kromatografijo smo analizirali njihove metilne estre celičnih maščobnih kislin (FA ME) in dimetilacetale (DMA). Primerjava sestave FA ME/ DMA je razkrila, da razlika v vsebnosti SCFA v gojišču povzroči izrazit kvantitativen učinek na deleže celičnih razvejanih maščobnih kislin in aldehidov le pri sevih vrste P. xylanivorans. Naša raziskava dokazuje, da lahko FA ME/DMA analizo učinkovito uporabljamo v kemotaksonomiji fenotipsko podobnih butirivibrijev z ustreznim ovrednotenjem vpliva gojišča.

• Keywords: mikrobiologija, anaerobne bakterije, vamp, Butyrivibrio, Pseudobutyrivibrio, FAME, DMA