Membrane changes associated with exposure of Pseudomonas putida to selected environmental pollutants and their possible roles in toxicity

Vodovnik, Maša (author), Bistan, Mirjana (author), Zorec, Maša (author), Marinšek-Logar, Romana (author)

A bacterial model system (Pseudomonas putida DSM 50026) was used in this research to assess potential effect of five selected chemically diverse environmental pollutants on cell membranes. Long chain fatty acid profiles of cultures exposed to environmentally relevant concentrations of atrazine (ATR), metolachlor (MET), pentachlorobiphenyl (PCB), hexachlorobenzene (HCB) and fluoranthene (FL), were analyzed and compared to non0exposed cultures. To assess sensitivity of membrane-based response, the impact of each toxicant on culture growth was also followed spectrophotometrically. Results revealed changes in fatty acid profiles when cells were exposed to PCB, HCB and FL in concentrations below the inhibitory levels. Moreover, the observed membrane responses were similar to the ones previously associated with adaption to some membrane-active compounds. On the other hand, exposure of cells to any of the two herbicides, ART or MET, did not induce any significant changes in fatty acid profiles. However, when combined with a commonly used fertilizer compound, NH[down]4NO[down]3, growth impairment was observed. Synergisitc effect of the two herbicides with NH[down]4NO[down]3, might be a consequence of changes in fatty acid profile increasing membrane fluidity, likely induced by NH[down]4+ ions.

• Language: English
• Keywords: mikrobiologija, toksikologija, bakterije, Pseudomonas putida, celične membrane, toksične snovi
• Work type: Not categorized
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Year: 2012
• Number of pages: Str. 83-88
• Numbering: Vol. 59, no. 1
• PID: 20.500.12556/RUL-36986
• UDC: 579:632.95.024
• ISSN on article: 1318-0207
• COBISS.SI-ID: 3024008
• Publication date in RUL: 10.07.2015

Record is a part of a journal

• Title: Acta chimica slovenica
• Shortened title: Acta chim. slov.
• Publisher: Slovensko kemijsko društvo, =Slovenian Chemical Society
• ISSN: 1318-0207
• COBISS.SI-ID: 14086149