Development of new microsatellite markers for Salvia officinalis L. and its potential use in conservation-genetic studies of narrow endemic Salvia brachyodon Vandas

Radosavljević, Ivan (avtor), Satović, Zlatko (avtor), Jakše, Jernej (avtor), Javornik, Branka (avtor), Greguraš, Danijela (avtor), Jug-Dujaković, Marija (avtor), Liber, Zlatko (avtor)

Nine new microsatellite markers (SSR) were isolated from Salvia officinalis L. A total of 125 alleles, with 8 to 21 alleles per locus, were detected in a natural population from the east Adriatic coast. The observed heterozygosity, expected heterozygosity, and polymorphic information content ranged from 0.46 to 0.83, 0.73 to 0.93 and 0.70 to 0.92, respectively. New microsatellite markers, as well as previously published markers, were tested for cross-amplification in Salvia brachyodon Vandas, a narrow endemic species known to be present in only two localities on the Balkan Peninsula. Out of 30 microsatellite markers tested on the natural S. brachyodon population, 15 were successfully amplified. To obtain evidence of recent bottleneck events in the populations of both species, observed genetic diversity (H$_E$) was compared to the expected genetic diversity at mutation-drift equilibrium (H$_{EQ}$) and calculated from the observed number of alleles using a two-phased mutation model (TPM). Recent bottleneck events were detected only in the S. brachyodon population. This result suggests the need to reconsider the current threat category of this endemic species.

• Jezik: Angleški jezik
• Ključne besede: salvia, common sage, laminaceae, microsatellites, microsatellite markers, conservation, cross-amplification, Dalmatian sage, genetic bottleneck, Lamiaceae, population genetics, short-tooth sage
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2012
• Št. strani: Str. 12082-12093
• Številčenje: Vol. 13, iss. 9
• PID: 20.500.12556/RUL-36382
• UDK: 577.2:633.88
• ISSN pri članku: 1661-6596
• DOI: 10.3390/ijms130912082
• COBISS.SI-ID: 7252089
• Datum objave v RUL: 10.07.2015

Gradivo je del revije

• Naslov: International journal of molecular sciences
• Skrajšan naslov: Int. j. mol. sci.
• Založnik: MDPI
• ISSN: 1661-6596
• COBISS.SI-ID: 36217605

Licence

• Licenca: CC BY 3.0, Creative Commons Priznanje avtorstva 3.0 Nedoločena
• Povezava: https://creativecommons.org/licenses/by/3.0/deed.sl
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• Začetek licenciranja: 24.09.2012

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: navadni žajbelj, dalmatinski žajbelj, mikrosatelitski markerji, mikrosateliti, populacije, Lamiaceae, genetska pestrost