Development of new microsatellite markers for Salvia officinalis L. and its potential use in conservation-genetic studies of narrow endemic Salvia brachyodon Vandas

Radosavljević, Ivan (author), Satović, Zlatko (author), Jakše, Jernej (author), Javornik, Branka (author), Greguraš, Danijela (author), Jug-Dujaković, Marija (author), Liber, Zlatko (author)

Nine new microsatellite markers (SSR) were isolated from Salvia officinalis L. A total of 125 alleles, with 8 to 21 alleles per locus, were detected in a natural population from the east Adriatic coast. The observed heterozygosity, expected heterozygosity, and polymorphic information content ranged from 0.46 to 0.83, 0.73 to 0.93 and 0.70 to 0.92, respectively. New microsatellite markers, as well as previously published markers, were tested for cross-amplification in Salvia brachyodon Vandas, a narrow endemic species known to be present in only two localities on the Balkan Peninsula. Out of 30 microsatellite markers tested on the natural S. brachyodon population, 15 were successfully amplified. To obtain evidence of recent bottleneck events in the populations of both species, observed genetic diversity (H$_E$) was compared to the expected genetic diversity at mutation-drift equilibrium (H$_{EQ}$) and calculated from the observed number of alleles using a two-phased mutation model (TPM). Recent bottleneck events were detected only in the S. brachyodon population. This result suggests the need to reconsider the current threat category of this endemic species.

• Language: English
• Keywords: salvia, common sage, laminaceae, microsatellites, microsatellite markers, conservation, cross-amplification, Dalmatian sage, genetic bottleneck, Lamiaceae, population genetics, short-tooth sage
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2012
• Number of pages: Str. 12082-12093
• Numbering: Vol. 13, iss. 9
• PID: 20.500.12556/RUL-36382
• UDC: 577.2:633.88
• ISSN on article: 1661-6596
• DOI: 10.3390/ijms130912082
• COBISS.SI-ID: 7252089
• Publication date in RUL: 10.07.2015

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1661-6596
• COBISS.SI-ID: 36217605

Licences

• License: CC BY 3.0, Creative Commons Attribution 3.0 Unported
• Link: https://creativecommons.org/licenses/by/3.0/deed.en
• Description: You are free to reproduce and redistribute the material in any medium or format. You are free to remix, transform, and build upon the material for any purpose, even commercially. You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use. You may not apply legal terms or technological measures that legally restrict others from doing anything the license permits.
• Licensing start date: 24.09.2012

Secondary language

• Language: Slovenian
• Keywords: navadni žajbelj, dalmatinski žajbelj, mikrosatelitski markerji, mikrosateliti, populacije, Lamiaceae, genetska pestrost