PCV2 and PCV3 genotyping in wild boars from Serbia

Nišavić, Jakov (author), Radalj, Andrea (author), Milić, Nenad (author), Prošić, Isidora (author), Živulj, Aleksandar (author), Benković, Damir (author), Vejnović, Branislav (author)

Porcine circoviruses 2 and 3 (PCV2 and PCV3) are known agents of diseases in domestic pigs and wild boars. PCV2 is an economically important pathogen causing porcine circovirus-associated diseases (PCVAD), while the recently discovered PCV3 is associated with similar disorders. Wild boars can serve as a PCV reservoir for domestic pigs, which is a particular risk for pig farms with low biosecurity. Reports of these infections in Serbia are sporadic, and this study was intended as a follow-up to an earlier study. Our aim was to assess the prevalence and genetic characteristics of PCVs circulating in wild boars in a region in north-eastern Serbia with extensive hunting areas. In our study of 103 samples, 17.48% tested positive for PCV2 and 15.53% for PCV3. The low co-infection rates in 2.94% of the PCR-positive samples, suggests these viruses circulate independently. PCV2 prevalence was lower than in our previous study (40.32% out of 124 samples), but the genetic stability of circulating strains was detected with a clear genotype shift towards PCV2d-2. Moreover, this is the first report of PCV3 occurrence in wild boar in Serbia, and the detected strains were grouped into two genotypes: PCV3-1 and PCV3-3c. The PCV3-1 sequences were clustered with German strains, indicating the prevalence of this genotype in Europe. However, no further geographical correlation could be established, as the PCV3-3c representative was separated within the cluster containing Chinese and Indian strains. Furthermore, there was no correlation between PCV positivity and pathological findings in the sampled animals indicating subclinical infection.

• Language: Slovenian
• Keywords: PCV2, PCV3, genotyping, PCR, wild boar
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Publication date: 01.01.2024
• Year: 2024
• Number of pages: Str. 263-269
• Numbering: Vol. 61, no. 4
• PID: 20.500.12556/RUL-168100
• UDC: 601.4:575.116.4: 599.731.111
• ISSN on article: 1580-4003
• DOI: 10.26873/SVR-1708-2024
• COBISS.SI-ID: 227302403
• Publication date in RUL: 28.03.2025

Record is a part of a journal

• Title: Slovenian veterinary research : the scientific journal of the Veterinary Faculty University of Ljubljana
• Shortened title: Slov. vet. res.
• Publisher: Veterinarska fakulteta, Založba Univerze v Ljubljani
• ISSN: 1580-4003
• COBISS.SI-ID: 108028416

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: English
• Title: Genotipizacija PCV2 in PCV3 pri divjih prašičih iz Srbije

Projects

• Funder: MESTD - Ministry of Education, Science and Technological Development of Republic of Serbia
• Project number: 451-03-66/2024-03/200143