Turbid optical phantoms using microspheres : a comprehensive guide to mixing protocols and optical property computation

Marin, Ana (author), Bürmen, Miran (author), Naglič, Peter (author)

Turbid optical phantoms play a crucial role in validating estimated optical properties across diverse applications, including medical imaging, surgical guidance, and photodynamic therapy. In this study, we discuss the benefits and limitations of both solid and liquid phantoms, emphasizing the precision in optical property adjustment offered by liquid phantoms. A detailed guideline for calculation of optical properties through Mie theory and corresponding implementation in a Monte Carlo-based light propagation model are provided. Finally, we introduce an open-source software tool designed to calculate the optical properties and provide a detailed mixing protocol. Additionally, we describe experimental setups for reflectance and transmittance measurements, examining the effects of microsphere size, size distribution, and solid content on reflectance, and identify an optimal size range for achieving spectrally resolvable features that are most useful for calibration or fine tuning of the Monte Carlo light propagation model. The manuscript includes examples of reflectance calibration and underscores the significance of accurate scattering phase function modeling in microsphere suspensions. This comprehensive guide aims to standardize the preparation and characterization of optical phantoms, enhancing their reliability and application across various optical fields.

• Language: English
• Keywords: optični fantomi, mikrosfere, monodisperzno, polidisperzno, odvisno sipanje, tekočina, suspenzija, optične lastnosti, sipanje, fazna funkcija, protokol mešanja, razširjanje svetlobe, Monte Carlo, Mie, odprto-kodni kalkulator
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Version of Record
• Year: 2025
• Number of pages: 11 str.
• Numbering: Vol. 74, art. 1002411
• PID: 20.500.12556/RUL-167349
• UDC: 535
• ISSN on article: 1557-9662
• DOI: 10.1109/TIM.2025.3527615
• COBISS.SI-ID: 222810627
• Publication date in RUL: 17.02.2025

MARIN, Ana, BÜRMEN, Miran and NAGLIČ, Peter, 2025, Turbid optical phantoms using microspheres : a comprehensive guide to mixing protocols and optical property computation. IEEE transactions on instrumentation and measurement [online]. 2025. Vol. 74, no. 1002411. [Accessed 15 April 2025]. DOI 10.1109/TIM.2025.3527615. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=167349

Record is a part of a journal

• Title: IEEE transactions on instrumentation and measurement
• Shortened title: IEEE trans. instrum. meas.
• Publisher: Institute of Electrical and Electronics Engineers
• ISSN: 1557-9662
• COBISS.SI-ID: 528591385

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: optical phantoms, microsphere, monodisperse, polydisperse, dependent scattering, liquid, suspension, optical properties, scattering, phase function, mixing protocol, light propagation, Monte Carlo, Mie, open-source calculator