Towards the application of nature's catalytic nanomachines : cellulosomes in 2nd generation biofuel production

Vodovnik, Maša (author), Lindič, Nataša (author)

Cellulosomes are highly efficient, complex multi-enzyme assemblies, predominantly found in anaerobic bacteria, which offer substantial potential for second-generation biofuel production through efficient lignocellulosic biomass degradation, thus reducing the need for costly pretreatments. Recent advances in cellulosome research have significantly contributed to developing more efficient consolidated bioprocessing (CBP) platforms for biofuel production. This review highlights the latest progress in designing cellulosomes for optimized enzyme synergy and substrate specificity, as well as advances in engineering cellulosome-producing whole-cell biocatalysts tailored for biofuel applications. Apart from recombinant approaches to the development of CBP platforms, metabolic engineering of cellulosome-producing strains (CPS) and co-culture systems that combine CPS with solvent-producing microbes are also discussed. Current challenges and future directions are outlined that emphasize the role of cellulosomes as powerful tools in advancing the efficiency of lignocellulosic biorefineries.

• Language: English
• Keywords: cellulosomes, designer cellulosomes, biofuels, consolidated bioprocessing, whole cell biocatalysts, strain engineering
• Work type: Article
• Typology: 1.02 - Review Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2025
• Number of pages: 14 str.
• Numbering: Vol. 79, art. 108523
• PID: 20.500.12556/RUL-167013
• UDC: 579
• ISSN on article: 1873-1899
• DOI: 10.1016/j.biotechadv.2025.108523
• COBISS.SI-ID: 224807939
• Publication date in RUL: 03.02.2025

Record is a part of a journal

• Title: Biotechnology advances
• Publisher: Elsevier
• ISSN: 1873-1899
• COBISS.SI-ID: 136899843

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: mikrobiologija, celulosomi, biogoriva

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P4-0097
• Name: Prehrana in mikrobna ekologija prebavil

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: P1-0048
• Name: Strukturna biologija