Exploring early DNA methylation alterations in type 1 diabetes : implications of glycemic control

Čugalj Kern, Barbara (avtor), Kovač, Jernej (avtor), Šket, Robert (avtor), Tesovnik, Tine (avtor), Jenko Bizjan, Barbara (avtor), Galhardo, Julia (avtor), Battelino, Tadej (avtor), Bratina, Nataša (avtor), Dovč, Klemen (avtor)

Background: Prolonged hyperglycemia causes diabetes-related micro- and macrovascular complications, which combined represent a significant burden for individuals living with diabetes. The growing scope of evidence indicates that hyperglycemia affects the development of vascular complications through DNA methylation. Methods: A genome-wide differential DNA methylation analysis was performed on pooled peripheral blood DNA samples from individuals with type 1 diabetes (T1D) with direct DNA sequencing. Strict selection criteria were used to ensure two age- and sex-matched groups with no clinical signs of chronic complications according to persistent mean glycated hemoglobin (HbA1c) values over 5 years: HbA1c<7% (N=10) and HbA1c>8% (N=10). Results: Between the two groups, 8385 differentially methylated CpG sites, annotated to 1802 genes, were identified. Genes annotated to hypomethylated CpG sites were enriched in 48 signaling pathways. Further analysis of key CpG sites revealed four specific regions, two of which were hypermethylated and two hypomethylated, associated with long non-coding RNA and processed pseudogenes. Conclusions: Prolonged hyperglycemia in individuals with T1D, who have no clinical manifestation of diabetes-related complications, is associated with multiple differentially methylated CpG sites in crucial genes and pathways known to be linked to chronic complications in T1D.

• Jezik: Angleški jezik
• Ključne besede: type 1 diabetes, glycemic control, DNA methylation, diabetes-related complications, long-read sequencing
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 10 str.
• Številčenje: Vol. 15, art. 1416433
• PID: 20.500.12556/RUL-166831
• UDK: 61
• ISSN pri članku: 1664-2392
• DOI: 10.3389/fendo.2024.1416433
• COBISS.SI-ID: 198843395
• Datum objave v RUL: 27.01.2025

Gradivo je del revije

• Naslov: Frontiers in endocrinology
• Založnik: Frontiers Media
• ISSN: 1664-2392
• COBISS.SI-ID: 3340154

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.