Clostridioides difficile is a Gram-positive, spore-producing, obligate anaerobic rod bacterium, that colonizes human gastrointestinal tract and is a common agent of gastrointestinal infections associated with antibiotic use. C. difficile can be classified by ribotyping. There are more than 900 known ribotypes. Some laboratoryes reported about patients infected with more than one ribotype. In Slovenia there is no such information available so the purpose of this thesis was to find possible infection with more than one C. difficile ribotype in a single patient. In the study, we tested 18 patients. From each patient we received two different primary plates and isolated 20 strains from each. In total, we isolated 720 isolates from all samples, which we classified into 15 different ribotypes. A mixed infection with three different ribotypes was present in one patient only. Our results suggest that mixed infections are present in the Maribor region but are very rare.
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