Gene immunotherapy of colon carcinoma with IL-2 and IL-12 using gene electrotransfer

Komel, Tilen (author), Omerzel, Maša (author), Kamenšek, Urška (author), Žnidar, Katarina (author), Lampreht Tratar, Urša (author), Kranjc Brezar, Simona (author), Dolinar, Klemen (author), Pirkmajer, Sergej (author), Serša, Gregor (author), Čemažar, Maja (author)

Gene immunotherapy has become an important approach in the treatment of cancer. One example is the introduction of genes encoding immunostimulatory cytokines, such as interleukin 2 and interleukin 12, which stimulate immune cells in tumours. The aim of our study was to determine the effects of gene electrotransfer of plasmids encoding interleukin 2 and interleukin 12 individually and in combination in the CT26 murine colon carcinoma cell line in mice. In the in vitro experiment, the pulse protocol that resulted in the highest expression of IL-2 and IL-12 mRNA and proteins was used for the in vivo part. In vivo, tumour growth delay and also complete response were observed in the group treated with the plasmid combination. Compared to the control group, the highest levels of various immunostimulatory cytokines and increased immune infiltration were observed in the combination group. Long-term anti-tumour immunity was observed in the combination group after tumour re-challenge. In conclusion, our combination therapy efficiently eradicated CT26 colon carcinoma in mice and also generated strong anti-tumour immune memory.

• Language: English
• Keywords: colon carcinoma, gene electrotransfer, gene immunotherapy, CT26 colon carcinoma, IL-2, IL-12, anti-tumour immune memory
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine; ZF - Faculty of Health Sciences
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 25 str.
• Numbering: Vol. 24, iss. 16, art. 12900
• PID: 20.500.12556/RUL-164842
• UDC: 602.6/.7
• ISSN on article: 1422-0067
• DOI: 10.3390/ijms241612900
• COBISS.SI-ID: 162334211
• Publication date in RUL: 13.11.2024

Record is a part of a journal

• Title: International journal of molecular sciences
• Shortened title: Int. j. mol. sci.
• Publisher: MDPI
• ISSN: 1422-0067
• COBISS.SI-ID: 2779162

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: rak debelega črevesa, elektroprenos genov, genska imunoterapija