Protein solutions are often used in the pharmaceutical industry, but it is important for their use to be stable and for the proteins to not aggregate into larger particles. In this master thesis, we have studied the properties of adsorbed molecular layers formed at the liquid-gas interface in protein solutions, or more specifically in our case, in biopharmaceuticals. To determine the viscoelastic properties, we used a surface rheometer that measures the response using a magnetized needle excited by an oscillating magnetic field of two coils. The same protein samples were subjected to controlled mechanical shaking and the resulting protein microparticles were analysed by flow imaging microscopy. We sought to determine whether there is a correlation between the viscoelastic properties of the surface adsorbed layer and the distribution of the mechanical stress-induced protein microparticles.
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