In the diploma thesis we investigated the influence of different growing conditions and preparations on the rooting of cuttings of seven genotypes of hemp (Cannabis sativa L.), analyzing the effects of two temperatures and preparations Clonex (Growth Technology Ltd.) and Mycorrhiza (Organics Nutrients) as well as the control (without preparation). After 21 days of incubation of the cuttings in propagators with different temperatures (23 °C and 26 °C), the survival of the cuttings was determined and rooting was evaluated. We then transplanted 4 cuttings from each treatment into 1.5 L pots and observed vegetative growth for 15 days. The results showed that a temperature of 23 °C allowed better rooting, survival and vegetative growth of the cuttings than a temperature of 26 °C. The Clonex preparation had a negative effect on the plants, causing a reduction in shoot length and vegetative growth, although the survival rate was not significantly different from that of the mycorrhizal preparation and the control. The mycorrhizal preparation improved survival and growth of cuttings at 23 °C, while the effects were not as pronounced at 26 °C. The age of the mother plant at the time of cutting was found to influence the vegetative growth of the cuttings, with younger plants performing better. When examining the stained roots under the microscope, we found some mycorrhizal hyphae, but not in large numbers, which means that it is difficult to talk about the influence of mycorrhiza on the measured variables. The study has shown the importance of choosing suitable conditions and preparations when preparing the cuttings and that further tests are needed, including studying the influence of different substrates and humidity on rooting, survival and vegetative growth
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