This thesis addresses the development and testing of a novel system for precise measurement of temperature gradients during electroporation processes within an electroporation cuvette. Electroporation, a technique utilizing strong electric fields to create transient pores in cell membranes for molecular introduction, requires accurate temperature control for optimal results. To enhance the accuracy and reproducibility of temperature measurements, an optical temperature sensor was employed in conjunction with a custom-designed, 3D-printed micromanipulator. This setup allows for three-dimensional positioning of the sensor, enabling temperature readings at arbitrary points within the cuvette. Experimental measurements revealed significant temperature variations at different locations within the cuvette, underscoring the importance of precise temperature monitoring and control during electroporation procedures. While some mechanical and structural limitations were observed in the 3D-printed components, the overall system demonstrated efficacy as a temperature measurement tool, as evidenced by the obtained results.
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