Razvoj metode za selekcijo haploidnih sevov kvasovke Saccharomyces cerevisiae, ki so sposobni kolonizirati človeško telo

Fink, Luka (author), Petrovič, Uroš (mentor)

Kvasovka Saccharomyces cerevisiae je v naravi vseprisotna in že vrsto tisočletij povezana s človekom. Prisotna je tako v živilih kot tudi na oziroma v človeku in še veliko drugih okoljih. Čeprav spada med varne mikroorganizme s statusom GRAS, lahko povzroči določene bolezni pri ljudeh s pomanjkljivim imunskih sistemom. Raziskovalci pripisujejo kvasovki S. cerevisiae sloves oportunističnega patogena. Naravni sevi imajo tako kot laboratorijski lastnosti, na podlagi katerih lahko ločimo vegetativne celice in spore. Z manipulacijo teh lastnosti lahko pripravimo haploidne seve za nadaljnje analize. V tej diplomski nalogi smo razvili metodo za selekcijo haploidnih sevov, ki so sposobni kolonizirati človeško telo. Metoda vključuje pripravo heterotalnih sevov in selekcijo haploidnih spor s toplotnim šokom. Ugotovili smo, da je optimalna temperatura toplotnega šoka 55 °C, ki omogoča obogatitev kulture vegetativnih celic in sporuliranih celic s sporami.

• Language: Slovenian
• Keywords: Saccharomyces cerevisiae, sporulacija, genetski inženiring, toplotni šok
• Work type: Bachelor thesis/paper
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Year: 2024
• PID: 20.500.12556/RUL-161375
• Publication date in RUL: 10.09.2024

Secondary language

• Language: English
• Title: Developing a method for the selection of haploid strains of yeast Saccharomyces cerevisiae that colonise the human body

Abstract

Yeast Saccharomyces cerevisiae is ubiquitous in nature and has been associated with humans for thousands of years. It is present in food as well as on or in humans and many other environments. Although it is classified among microorganisms with the GRAS status, it can cause certain diseases in people with compromised immune systems. Researchers identify S. cerevisiae as an opportunistic pathogen. Both, natural and laboratory strains have properties that allow the differentiation of vegetative cells and spores. By manipulating these properties, haploid strains can be prepared for further analysis. In this diploma work, we developed a method for the selection of haploid strains capable of colonizing the human body. The method involves the preparation of heterothallic strains and the selection of haploid spores with heat shock. We identified 55°C as the optimal heat shock temperature, which enriches the culture consisting of vegetative cells and sporulated cells with spores.

• Keywords: Saccharomyces cerevisiae, sporulation, genetic engineering, heat shock