Učinkovitost bio bariere na higieno perila med nizko temperaturnim pranjem

Tkachenko, Julia (avtor), Tomšič, Brigita (mentor), Fink, Rok (komentor)

Namen diplomskega dela je bil razviti protimikrobne krpice za zagotavljanje higiene pri nizkotemperaturnem pranju ter določiti vpliv višanja števila protimikrobnih krpic v pralni kopeli na redukcijo rasti bakterij, gram-negativne Escherichia coli (E. coli) in gram-pozitivne Staphylococcus aureus (S. aureus). V eksperimentalnem delu je bila 100-odstotna viskozna vlaknovina obdelana s protimikrobnim sredstvom 3-(trimetoksisilil)-propildimetilalkil-amonijevim kloridom (SiQAS) po impregnirnem postopku. Na podlagi vrstične elektronske mikroskopije (SEM) in infrardeče spektroskopije s Fourierjevo transformacijo (FT-IR) so bile določene morfološke in kemijske spremembe apretiranih viskoznih vlaken, s testom z bromofenol modrim (BPB) reagentom pa določena primernost tržnega pralnega sredstva Ariel Color+ in Perwoll za ohranjanje aktivnosti bio-bariere med postopkom pranja. Ustreznost higiene gospodinjskega pranja pri temperaturi 30 °C ob prisotnosti enega in treh apretiranih vzorcev viskozne vlaknovine je bila določena s standardno metodo SIST EN 17658:2021. Nanos SiQAS je na viskoznih vlaknih ustvaril enakomeren film, rahlo povečal hrapavost in nekoliko zabrisal značilne vzdolžne linije viskoznih vlaken. Rezultati FT-IR analize so potrdili prisotnost protimikrobno aktivne kvarterne amonijeve skupine z dolgo alkilno verigo sredstva SiQAS na viskoznih vlaknih. Pranje s Perwollom je bolje ohranilo aktivnost bio-barriere kot pranje z Ariel Color+, saj je po pranju povzročilo le rahlo nižjo adsorpcijo BPB reagenta na apretirana viskozna vlakna in ohranilo intenzivnost modrega obarvanja. Kljub temu dodatek apretiranih vzorcev viskozne vlaknovine ni zagotovil ustrezne higiene pranja pri nizkih temperaturah, saj ni odrazil učinkovite redukcije bakterij E. coli in S. aureus. Dodatek treh apretiranih vzorcev viskozne vlaknovine v pralno kopel je sicer nekoliko zmanjšal mikrobno obremenitev med nizkotemperaturnim pranjem, saj je za 0,77 in 1,57 log CFU/ml preprečil navzkrižno kontaminacijo z E. coli in S. aureus ter hkrati nekoliko zmanjšal število teh bakterij v odpadni vodi za 0,66 (S. aureus) in 0,16 log CFU/ml (E. coli). Za dosego ustrezne higiene nizkotemperaturnega pranja s SiQAS krpicami je potrebna nadaljnja optimizacija postopka.

• Jezik: Slovenski jezik
• Ključne besede: nega tekstilij, gospodinjsko pranje, nizkotemperaturno pranje, higiena pranja, protimikrobna apretura
• Vrsta gradiva: Diplomsko delo/naloga
• Organizacija: NTF - Naravoslovnotehniška fakulteta
• Leto izida: 2024
• PID: 20.500.12556/RUL-161031
• Datum objave v RUL: 06.09.2024

Sekundarni jezik

• Jezik: Angleški jezik
• Naslov: Effectiveness of bio barrier on laundry hygiene during low temperature washing

Izvleček

The aim of the thesis was to develop antimicrobial wipes to ensure hygiene when washing at low temperatures and to determine the effect of increasing the number of antimicrobial wipes in the wash bath on the reduction of bacterial growth of gramme-negative Escherichia coli (E. coli) and gramme-positive Staphylococcus aureus (S. aureus). A nonwoven fabric made of 100% viscose (CV) was treated with the antimicrobial agent 3-(trimethoxysilyl)-propyldimethylalkylammonium chloride (SiQAS) using an impregnation process. Scanning electron microscopy (SEM) and Fourier transform infrared spectroscopy (FT-IR) were used to determine the morphological and chemical properties of the finished CV wipes, and a bromophenol blue (BPB) reagent test was used to determine the suitability of the commercially available detergents Ariel Colour+ and Perwoll to maintain bio-barrier activity during the washing process. The adequacy of household laundry hygiene when washing at 30 °C in the presence of one and three finished CV wipes was determined according to the standard method SIST EN 17658:2021. The application of SiQAS formed a uniform film on the CV fibres, slightly increased the roughness and slightly blurred the characteristic longitudinal lines of the viscose fibres. The results of FT-IR analysis confirmed the presence of an antimicrobially active quaternary ammonium group with a long alkyl chain of SiQAS on the CV fibres. Washing with Perwoll preserved the activity of the bio-barrier better than washing with Ariel Colour+, as it only led to a slightly lower adsorption of the BPB reagent on the treated CV fibres after washing and maintained the intensity of the blue colouration. Nevertheless, the addition of treated CV wipes did not ensure sufficient washing hygiene at low temperatures as it did not reflect the effective reduction of E. coli and S. aureus bacteria. The addition of three treated CV wipes to the wash bath slightly reduced the microbial load during low temperature washing, as it prevented the transfer of E. coli and S. aureus by 0.77 and 1.57 log CFU/mL, respectively, while slightly reducing the number of these bacteria in the effluent by 0.66 (S. aureus) and 0.16 log CFU/mL (E. coli). Further optimisation of the process is required to achieve adequate hygiene in low-temperature washing with SiQAS treated wipes.

• Ključne besede: textile care, household laundry, low-temperature washing, washing hygiene, antimicrobial finish