Understanding and investigating interactions between cells and differently laser-treated metal surfaces is crucial for the development of innovative biomaterials with potential applications in medical settings. In this master's thesis, we focused on investigating interactions between human osteosarcoma cells MG-63 and various laser-textured and 3D laser-printed patterns of 316L steel. Cell viability on differently laser-textured metal surfaces was assessed after 24, 72, and 168 hours of incubation using differential staining with Hoechst and propidium iodide dyes. To evaluate cytotoxicity and cellular physiological status on various laser-textured surfaces, we utilized resazurin dye for measuring metabolic activity and neutral red (NR) dye for assessing lysosomal integrity. Cell morphology was examined using scanning electron microscopy. Results indicate differences in cell viability, metabolic activity, lysosomal integrity, and cell morphology among various samples. We hypothesize that multiple factors, including the cracking of oxide layers, leaching of metal ions, and diverse surface structures of the samples, contribute to the cytotoxicity observed. We demonstrated that cell morphology is dependent on the substrate on which cells grow and, together with cytotoxicity, reflects the biocompatibility of materials. This study provides initial insights into the interaction between MG-63 cells and differently laser-treated samples, which could potentially be utilized for medical purposes.
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