New breeding methods using CRISPR/Cas technology have proven to be a potential solution to the increasing demands in agriculture. Plants with higher yields, better quality and resilience to environmental factors and pathogens can be obtained faster and with less effort compared to traditional breeding methods. The process of plant modification begins with the selection of the target gene and the design of the guide RNA (sgRNA), followed by plant transformation and regeneration, and then validation and confirmation of mutations. These advantages enable faster development of new varieties, which significantly contributes to improving food security. Additionally, CRISPR/Cas9 is more accessible and cost-effective compared to some traditional gene editing methods, meaning more opportunities for crop improvement even in less developed regions of the world. Despite the challenges, CRISPR/Cas9 technology holds significant potential for enhancing agricultural productivity and reducing global food insecurity.
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