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Effect of castration method on porcine skeletal muscle fiber traits and transcriptome profiles
ID
Poklukar Žnidaršič, Klavdija
(
Avtor
),
ID
Erbežnik, Anja
(
Avtor
),
ID
Fazarinc, Gregor
(
Avtor
),
ID
Kress, Kevin
(
Avtor
),
ID
Batorek Lukač, Nina
(
Avtor
),
ID
Škrlep, Martin
(
Avtor
),
ID
Stefanski, Volker
(
Avtor
),
ID
Čandek-Potokar, Marjeta
(
Avtor
),
ID
Vrecl, Milka
(
Avtor
)
PDF - Predstavitvena datoteka,
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(4,39 MB)
MD5: 9FE27A8B790F38D696D081D232C54E76
URL - Izvorni URL, za dostop obiščite
https://www.sciencedirect.com/science/article/pii/S2451943X24000504?via%3Dihub
Galerija slik
Izvleček
This study examined the effects of immunocastration and surgical castration on the histomorphometric and transcriptome traits of the porcine skeletal muscle. We hypothesized that the differences in duration of androgen deprivation resulting from different castration methods influence skeletal muscle biology in a muscle-specific manner. This was tested by analyzing samples of m. longissimus dorsi (LD) and m. semispinalis capitis (SSC) from immunocastrated (IC; n = 12), entire male (EM; n = 12), and surgically castrated (SC; n = 12) pigs using enzyme/immunohistochemical classification and histomorphometric analysis of myofibers, quantitative PCR, and RNA sequencing. The results confirmed the distinctive histomorphometric profiles of LD and SSC and the castration method related muscle-specific effects at the histomorphometric and transcriptome levels. Long-term androgen deficiency (surgical castration) significantly reduced the proportion of fast-twitch type IIa myofibers in LD (P < 0.05), whereas short-term androgen deprivation (immunocastration) reduced the cross-sectional area of oxidative type I myofibers in SSC (P < 0.05). At the transcriptional level, glycolytic LD adapted to long- and short-term androgen deprivation by upregulating genes controlling myoblast proliferation and differentiation to maintain fiber size. In contrast, increased protein degradation through the ubiquitin ligase-mediated atrophy pathway (significantly increased TRIM63 and FBXO32 expression; P < 0.05) could underly reduced crosssectional area of type I myofibers in the oxidative SSC in IC. Potential candidate genes (HK2, ARID5B, SERPINE1, and SCD) linked to specific metabolic profiles and meat quality traits were also identified in IC, providing a foundation for studying the effects of immunocastration on skeletal muscle fiber and carcass/meat quality traits.
Jezik:
Angleški jezik
Ključne besede:
sceletal muscle
,
Logissimus dorsi
,
Semispinalis capitis
,
castration method
,
genne expression
,
pig
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
VF - Veterinarska fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2024
Št. strani:
13 str.
Številčenje:
Vol. 25, art. 100383
PID:
20.500.12556/RUL-160055
UDK:
636.4.09:611
ISSN pri članku:
2451-943X
DOI:
10.1016/j.vas.2024.100383
COBISS.SI-ID:
203362563
Datum objave v RUL:
13.08.2024
Število ogledov:
150
Število prenosov:
43
Metapodatki:
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Objavi na:
Gradivo je del revije
Naslov:
Veterinary and animal science
Založnik:
Elsevier Ltd.
ISSN:
2451-943X
COBISS.SI-ID:
526790681
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Začetek licenciranja:
13.08.2024
Projekti
Financer:
EC - European Commission
Program financ.:
Slovenian Ministry for Agriculture in the frame of ERA NET SusAn SuSi (631-10/2015/7; Sustainability in pork production with immunocastration)
Številka projekta:
631-10/2015/7
Financer:
ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:
P4-0133
Naslov:
Trajnostno kmetijstvo
Financer:
ARIS - Javna agencija za znanstvenoraziskovalno in inovacijsko dejavnost Republike Slovenije
Številka projekta:
P4-0053
Naslov:
Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih
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