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Effect of castration method on porcine skeletal muscle fiber traits and transcriptome profiles
ID
Poklukar Žnidaršič, Klavdija
(
Author
),
ID
Erbežnik, Anja
(
Author
),
ID
Fazarinc, Gregor
(
Author
),
ID
Kress, Kevin
(
Author
),
ID
Batorek Lukač, Nina
(
Author
),
ID
Škrlep, Martin
(
Author
),
ID
Stefanski, Volker
(
Author
),
ID
Čandek-Potokar, Marjeta
(
Author
),
ID
Vrecl, Milka
(
Author
)
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Abstract
This study examined the effects of immunocastration and surgical castration on the histomorphometric and transcriptome traits of the porcine skeletal muscle. We hypothesized that the differences in duration of androgen deprivation resulting from different castration methods influence skeletal muscle biology in a muscle-specific manner. This was tested by analyzing samples of m. longissimus dorsi (LD) and m. semispinalis capitis (SSC) from immunocastrated (IC; n = 12), entire male (EM; n = 12), and surgically castrated (SC; n = 12) pigs using enzyme/immunohistochemical classification and histomorphometric analysis of myofibers, quantitative PCR, and RNA sequencing. The results confirmed the distinctive histomorphometric profiles of LD and SSC and the castration method related muscle-specific effects at the histomorphometric and transcriptome levels. Long-term androgen deficiency (surgical castration) significantly reduced the proportion of fast-twitch type IIa myofibers in LD (P < 0.05), whereas short-term androgen deprivation (immunocastration) reduced the cross-sectional area of oxidative type I myofibers in SSC (P < 0.05). At the transcriptional level, glycolytic LD adapted to long- and short-term androgen deprivation by upregulating genes controlling myoblast proliferation and differentiation to maintain fiber size. In contrast, increased protein degradation through the ubiquitin ligase-mediated atrophy pathway (significantly increased TRIM63 and FBXO32 expression; P < 0.05) could underly reduced crosssectional area of type I myofibers in the oxidative SSC in IC. Potential candidate genes (HK2, ARID5B, SERPINE1, and SCD) linked to specific metabolic profiles and meat quality traits were also identified in IC, providing a foundation for studying the effects of immunocastration on skeletal muscle fiber and carcass/meat quality traits.
Language:
English
Keywords:
sceletal muscle
,
Logissimus dorsi
,
Semispinalis capitis
,
castration method
,
genne expression
,
pig
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
VF - Veterinary Faculty
Publication status:
Published
Publication version:
Version of Record
Year:
2024
Number of pages:
13 str.
Numbering:
Vol. 25, art. 100383
PID:
20.500.12556/RUL-160055
UDC:
636.4.09:611
ISSN on article:
2451-943X
DOI:
10.1016/j.vas.2024.100383
COBISS.SI-ID:
203362563
Publication date in RUL:
13.08.2024
Views:
146
Downloads:
43
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Record is a part of a journal
Title:
Veterinary and animal science
Publisher:
Elsevier Ltd.
ISSN:
2451-943X
COBISS.SI-ID:
526790681
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Licensing start date:
13.08.2024
Projects
Funder:
EC - European Commission
Funding programme:
Slovenian Ministry for Agriculture in the frame of ERA NET SusAn SuSi (631-10/2015/7; Sustainability in pork production with immunocastration)
Project number:
631-10/2015/7
Funder:
ARIS - Slovenian Research and Innovation Agency
Project number:
P4-0133
Name:
Trajnostno kmetijstvo
Funder:
ARIS - Slovenian Research and Innovation Agency
Project number:
P4-0053
Name:
Endokrini, imunski in encimski odzivi pri zdravih in bolnih živalih
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