The gene complex Fomes fomentarius sensu lato consists of two major genetic lineages, namely line A also named Fomes fomentarius sensu stricto, which is divided into lineages A1 and A2, and lineage B or Fomes inzengae, which is divided into lineages B1 and B2. It is highly unlikely that these lineages can be distinguished solely on the basis of morphological characteristics alone, but comparing the sequences of the ITS region, which is a commonly used genetic marker for fungi, offers a simple and reliable way of genetic identification. So as to identify the genetic lineages of the mentioned complex in Slovenia, we isolated the mycelium of the purposely collected fruiting bodies in pure cultures and by DNA nuclear ITS region and tef1 region analysis method attained nucleotide sequences of a whole ITS region and partial tef1 region. Sequence comparison confirmed the presence of genetic lineages A2 and B2 in Slovenia. By plotting the sample locations, we ascertained that the genetic lineages present in Slovenia do not have a specific pattern of distribution and that they both appear all over each of the sampled area. Our results were consistent with those found in the literature, which are revealing the presence of both lineages in Europe with no distinct geographical distribution pattern.
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