Solution-state structure of a long-loop G-quadruplex formed within promoters of Plasmodium falciparum B var genes

Juribašić Kulcsár, Marina (avtor), Gabelica, Valérie (avtor), Plavec, Janez (avtor)

We report the high-resolution NMR solution-state structure of an intramolecular G-quadruplex with a diagonal loop of ten nucleotides. The G-quadruplex is formed by a 34-nt DNA sequence, d[CAG$_3$T$_2$A$_2$G$_3$TATA$_2$CT$_3$AG$_4$T$_2$AG$_3$T$_2$], named UpsB-Q-1. This sequence is found within promoters of the var genes of Plasmodium falciparum, which play a key role in malaria pathogenesis and evasion of the immune system. The [3+1]-hybrid G-quadruplex formed under physiologically relevant conditions exhibits a unique equilibrium between two structures, both stabilized by base stacking and non-canonical hydrogen bonding. Unique equilibrium of the two closely related 3D structures originates from a North-South repuckering of deoxyribose moiety of residue T27 in the lateral loop. Besides the 12 guanines involved in three G-quartets, most residues in loop regions are involved in interactions at both G-quartet-loop interfaces.

• Jezik: Angleški jezik
• Ključne besede: G-quadruplex, nuclear magnetic resonance, long loop, loop interactions, Plasmodium falciparum
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 11 str.
• Številčenje: Vol. 30, iss. 36, art. e202401190
• PID: 20.500.12556/RUL-159456
• UDK: 577
• ISSN pri članku: 1521-3765
• DOI: 10.1002/chem.202401190
• COBISS.SI-ID: 201336579
• Datum objave v RUL: 10.07.2024

Gradivo je del revije

• Naslov: Chemistry : a European journal
• Skrajšan naslov: Chemistry
• Založnik: Wiley, Chemistry Europe
• ISSN: 1521-3765
• COBISS.SI-ID: 21813253

Licence

• Licenca: CC BY-NC 4.0, Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc/4.0/deed.sl
• Opis: Licenca Creative Commons, ki prepoveduje komercialno uporabo, vendar uporabniki ne rabijo upravljati materialnih avtorskih pravic na izpeljanih delih z enako licenco.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: biokemija, g-kvadrupleksi, NMR, 3D strukture, malarija