Immobilization of ▫$His_6$▫-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes

Šketa, Borut (avtor), Galman, James L. (avtor), Turner, Nicholas J. (avtor), Žnidaršič Plazl, Polona (avtor)

Enzyme immobilization is one of the essential tools that enables bioprocess intensification as it facilitates reuse of enzyme. Nanomaterials can be used as supports for enzyme immobilization because of their high surface to volume ratio. In his work a designed biocompatible non-woven nanofiber was used as a support for hexahistidine tagged amine transaminase immobilization in a microreactor. Four different nanofibers were tested and the one with surface immobilized Cu$^{2+}$ ions was proven to be the best. Additionally, direct immobilization of overexpressed enzymes in E. coli cell lysate was performed and yielded loads up to 1088 U mL$^{-1}$. The highest turnover number reached within the indicated time was 7.23 10$^6$.

• Jezik: Angleški jezik
• Ključne besede: enzyme immobilization, amine transaminase, microreactor, membrane
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FKKT - Fakulteta za kemijo in kemijsko tehnologijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: Str. 46-55
• Številčenje: Vol. 83
• PID: 20.500.12556/RUL-159298
• UDK: 66.098
• ISSN pri članku: 1871-6784
• DOI: 10.1016/j.nbt.2024.06.005
• COBISS.SI-ID: 200710147
• Datum objave v RUL: 05.07.2024

Gradivo je del revije

• Naslov: New biotechnology
• Založnik: Elsevier
• ISSN: 1871-6784
• COBISS.SI-ID: 30012933

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: imobilizacija encimov, amin transaminaze, mikroreaktor, membrana