Immobilization of ▫$His_6$▫-tagged amine transaminases in microreactors using functionalized nonwoven nanofiber membranes

Šketa, Borut (author), Galman, James L. (author), Turner, Nicholas J. (author), Žnidaršič Plazl, Polona (author)

Enzyme immobilization is one of the essential tools that enables bioprocess intensification as it facilitates reuse of enzyme. Nanomaterials can be used as supports for enzyme immobilization because of their high surface to volume ratio. In his work a designed biocompatible non-woven nanofiber was used as a support for hexahistidine tagged amine transaminase immobilization in a microreactor. Four different nanofibers were tested and the one with surface immobilized Cu$^{2+}$ ions was proven to be the best. Additionally, direct immobilization of overexpressed enzymes in E. coli cell lysate was performed and yielded loads up to 1088 U mL$^{-1}$. The highest turnover number reached within the indicated time was 7.23 10$^6$.

• Language: English
• Keywords: enzyme immobilization, amine transaminase, microreactor, membrane
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FKKT - Faculty of Chemistry and Chemical Technology
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: Str. 46-55
• Numbering: Vol. 83
• PID: 20.500.12556/RUL-159298
• UDC: 66.098
• ISSN on article: 1871-6784
• DOI: 10.1016/j.nbt.2024.06.005
• COBISS.SI-ID: 200710147
• Publication date in RUL: 05.07.2024

Record is a part of a journal

• Title: New biotechnology
• Publisher: Elsevier
• ISSN: 1871-6784
• COBISS.SI-ID: 30012933

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: imobilizacija encimov, amin transaminaze, mikroreaktor, membrana