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Size matters : rethinking Hertz model interpretation for cell mechanics using AFM
ID
Mendová, Katarina
(
Avtor
),
ID
Otáhal, Martin
(
Avtor
),
ID
Drab, Mitja
(
Avtor
),
ID
Daniel, Matej
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(636,83 KB)
MD5: EB6B928B951201BC610B3DFCFC2DE0BF
URL - Izvorni URL, za dostop obiščite
https://www.mdpi.com/1422-0067/25/13/7186
Galerija slik
Izvleček
Cell mechanics are a biophysical indicator of cell state, such as cancer metastasis, leukocyte activation, and cell cycle progression. Atomic force microscopy (AFM) is a widely used technique to measure cell mechanics, where the Young modulus of a cell is usually derived from the Hertz contact model. However, the Hertz model assumes that the cell is an elastic, isotropic, and homogeneous material and that the indentation is small compared to the cell size. These assumptions neglect the effects of the cytoskeleton, cell size and shape, and cell environment on cell deformation. In this study, we investigated the influence of cell size on the estimated Young’s modulus using liposomes as cell models. Liposomes were prepared with different sizes and filled with phosphate buffered saline (PBS) or hyaluronic acid (HA) to mimic the cytoplasm. AFM was used to obtain the force indentation curves and fit them to the Hertz model. We found that the larger the liposome, the lower the estimated Young’s modulus for both PBS-filled and HA-filled liposomes. This suggests that the Young modulus obtained from the Hertz model is not only a property of the cell material but also depends on the cell dimensions. Therefore, when comparing or interpreting cell mechanics using the Hertz model, it is essential to account for cell size.
Jezik:
Angleški jezik
Ključne besede:
atomic force microscopy (AFM)
,
cell mechanics
,
cell stiffness
,
Hertz contact model
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
FE - Fakulteta za elektrotehniko
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2024
Št. strani:
12 str.
Številčenje:
Vol. 25, iss. 13, [article no.] 7186
PID:
20.500.12556/RUL-159192
UDK:
620.3
ISSN pri članku:
1422-0067
DOI:
10.3390/ijms25137186
COBISS.SI-ID:
200434179
Datum objave v RUL:
02.07.2024
Število ogledov:
157
Število prenosov:
19
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Objavi na:
Gradivo je del revije
Naslov:
International journal of molecular sciences
Skrajšan naslov:
Int. j. mol. sci.
Založnik:
MDPI
ISSN:
1422-0067
COBISS.SI-ID:
2779162
Licence
Licenca:
CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
Povezava:
http://creativecommons.org/licenses/by/4.0/deed.sl
Opis:
To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.
Sekundarni jezik
Jezik:
Slovenski jezik
Ključne besede:
mikroskopija na atomsko silo (AFM)
,
celična mehanika
,
togost celic
,
Hertzov kontaktni model
Projekti
Financer:
Drugi - Drug financer ali več financerjev
Program financ.:
Czech Science Foundation
Številka projekta:
SGS22/149/OHK2/3T/12
Naslov:
Research and development of methods and applications for diagnostic and therapeutic biomechanics
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