DNA barcoding insufficiently identifies European wild bees (Hymenoptera, Anthophila) due to undefined species diversity, genus-specific barcoding gaps and database errors

Šet, Janko (avtor), Šturm, Rok (avtor), Koderman, Blaž (avtor), Bevk, Danilo (avtor), Gogala, Andrej (avtor), Kutnjak, Denis (avtor), Čandek, Klemen (avtor), Gregorič, Matjaž (avtor)

Recent declines in insect abundances, especially populations of wild pollinators, pose a threat to many natural and agricultural ecosystems. Traditional species monitoring relies on morphological character identification and is inadequate for efficient and standardized surveys. DNA barcoding has become a standard approach for molecular identification of organisms, aiming to overcome the shortcomings of traditional biodiversity monitoring. However, its efficacy depends on the completeness of reference databases. Large DNA barcoding efforts are (almost entirely) lacking in many European countries and such patchy data limit Europe-wide analyses of precisely how to apply DNA barcoding in wild bee identification. Here, we advance towards an effective molecular identification of European wild bees. We conducted a high-effort survey of wild bees at the junction of central and southern Europe and DNA barcoded all collected morphospecies. For global analyses, we complemented our DNA barcode dataset with all relevant European species and conducted global analyses of species delimitation, general and genus-specific barcoding gaps and examined the error rate in DNA data repositories. We found that (i) a sixth of all specimens from Slovenia could not be reliably identified, (ii) species delimitation methods show numerous systematic discrepancies, (iii) there is no general barcoding gap across all bees and (iv) the barcoding gap is genus specific, but only after curating for errors in DNA data repositories. Intense sampling and barcoding efforts in underrepresented regions and strict curation of DNA barcode repositories are needed to enhance the use of DNA barcoding for the identification of wild bees.

• Jezik: Angleški jezik
• Ključne besede: insect pollinators, wild bees, DNA, biodiversity, databases, insect decline, pollinator, species delimitation, taxonomic impediment
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: BF - Biotehniška fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: 31 str.
• Številčenje: Vol. 24, iss. 5, art. e13953
• PID: 20.500.12556/RUL-158319
• UDK: 595.799:57.088.7:631.527.82
• ISSN pri članku: 1755-098X
• DOI: 10.1111/1755-0998.13953
• COBISS.SI-ID: 191613699
• Datum objave v RUL: 05.06.2024

Gradivo je del revije

• Naslov: Molecular ecology resources
• Skrajšan naslov: Mol. ecol. resour.
• Založnik: Wiley
• ISSN: 1755-098X
• COBISS.SI-ID: 5711737

Licence

• Licenca: CC BY-NC 4.0, Creative Commons Priznanje avtorstva-Nekomercialno 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc/4.0/deed.sl
• Opis: Licenca Creative Commons, ki prepoveduje komercialno uporabo, vendar uporabniki ne rabijo upravljati materialnih avtorskih pravic na izpeljanih delih z enako licenco.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: žuželke opraševalci, divje čebele, DNA, biološka raznovrstnost, zbirke podatkov