Insights into the immunological description of cryoglobulins with regard to detection and characterization in Slovenian rheumatological patients

Ogrič, Manca (avtor), Švec, Tinka (avtor), Mrak Poljšak, Katjuša (avtor), Lakota, Katja (avtor), Podovšovnik, Eva (avtor), Kolopp-Sarda, Marie Nathalie (avtor), Hočevar, Alojzija (avtor), Čučnik, Saša (avtor)

The detection of cryoglobulins (CG) used to diagnose cryoglobulemic vasculitis requires strict adherence to protocol, with emphasis on the preanalytical part. Our main objectives were to introduce a more sensitive and specific protocol for the detection of CG and to characterize CG in Slovenian patients diagnosed with cryoglobulinemic vasculitis, other vasculitides, connective tissue diseases or non-rheumatic diseases examined at the Department of Rheumatology (University Medical Centre Ljubljana). Samples were routinely analyzed for the presence of CG with the protocol using the Folin-Ciocalteu reagent. In the newly introduced protocol, the type of CG was determined by immunofixation on visually observed positive samples, and the concentration of CG in the cryoprecipitate and rheumatoid factor (RF) activity were measured by nephelometry. RF, C3c and C4 were measured in the patients` serum, and decision tree analysis was performed using all results. The agreement between negative and positive results between the two protocols was 86%. Of the 258 patient samples tested, we found 56 patients (21.7%) with positive CG (37.5% - type II, 62.5% - type III). The RF activity was observed in 21.4% of CG positive subjects. The median concentration of type II CG was significantly higher than that of type III CG (67.4 mg/L vs. 45.0 mg/L, p = 0.037). Patients with type II had lower C4 concentrations and higher RF activity compared to patients with type III CG. In the decision tree, C4 was the strongest predictor of cryoglobulinemia in patients. With the newly implemented protocol, we were able to improve the detection and quantification of CG in the samples of our rheumatology patients and report the results to adequately support clinicians.

• Jezik: Angleški jezik
• Ključne besede: cryoglobulins, cryoglobulinemic vasculitis, rheumatoid factor activity, complement components, cryoglobulin detection, pre-analytical phase
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta ; FFA - Fakulteta za farmacijo
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: Str. 185–196
• Številčenje: Vol. 72, iss. 2
• PID: 20.500.12556/RUL-155807
• UDK: 616-097
• ISSN pri članku: 0257-277X
• DOI: 10.1007/s12026-023-09434-9
• COBISS.SI-ID: 171557379
• Datum objave v RUL: 19.04.2024

Gradivo je del revije

• Naslov: Immunologic research
• Skrajšan naslov: Immunol. res.
• Založnik: Springer Nature
• ISSN: 0257-277X
• COBISS.SI-ID: 514874649

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: krioglobulini, krioglobulinemični vaskulitis, aktivnost revmatoidnega faktorja, komponente komplementa, detekcija krioglobulina, predanalitična faza

Projekti

• Financer: Drugi - Drug financer ali več financerjev
• Program financ.: University Medical Centre Ljubljana, Tertiary project

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P3-0314
• Naslov: Sistemske avtoimunske bolezni