Food and wood residues are a great source of raw materials for environmentally
friendly biotechnological processes. In that purpose in the master thesis, we tried to
exploit the potential of bio-residues and to obtain increased amounts of released
polyphenols with four fungal species, natural decomposers and molds in a solid-state
cultivation (SSF) process. Polyphenols are natural compounds that show great
applicative potential in food, pharmaceutical and cosmetic industry, mainly due to
their antioxidant capacity. Our selected fungal species were A. niger, R. arrhizus, P.
ostreatus and A. flavus and 6 different substrates were used to carry out SSF: used
mint, green and fruit tea bags, coffee grounds, fir bran from cores and bark.
Cultivation lasted for 15 days, with two sampling times. Extraction of the samples was
achieved by 3 techniques: magnetic stirrer, ultrasound sonication and accelerated
solvent extraction, simultaneously examining which is the most efficient and
economically advantageous. Resulting that all are comparable and effective. The
extracts were analysed gravimetrically and spectrophotometrically, the profile of the
present compounds was examined by high-performance liquid chromatography.
Results suggest that SSF process was successfully carried out and higher amounts of
polyphenols can be extracted from the bio-residues with suitable fungi.
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