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Segmentation strategy of de novo designed four-helical bundles expands protein oligomerization modalities for cell regulation
ID
Merljak, Estera
(
Author
),
ID
Malovrh, Benjamin
(
Author
),
ID
Jerala, Roman
(
Author
)
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https://www.nature.com/articles/s41467-023-37765-6
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Abstract
Protein–protein interactions govern most biological processes. New protein assemblies can be introduced through the fusion of selected proteins with di/oligomerization domains, which interact specifically with their partners but not with other cellular proteins. While four-helical bundle proteins (4HB) have typically been assembled from two segments, each comprising two helices, here we show that they can be efficiently segmented in various ways, expanding the number of combinations generated from a single 4HB. We implement a segmentation strategy of 4HB to design two-, three-, or four-chain combinations for the recruitment of multiple protein components. Different segmentations provide new insight into the role of individual helices for 4HB assembly. We evaluate 4HB segmentations for potential use in mammalian cells for the reconstitution of a protein reporter, transcriptional activation, and inducible 4HB assembly. Furthermore, the implementation of trimerization is demonstrated as a modular chimeric antigen receptor for the recognition of multiple cancer antigens.
Language:
English
Keywords:
molecular engineering
,
synthetic biology
Work type:
Article
Typology:
1.01 - Original Scientific Article
Organization:
MF - Faculty of Medicine
Publication status:
Published
Publication version:
Version of Record
Year:
2023
Number of pages:
12 str.
Numbering:
Vol. 14, art. 1995
PID:
20.500.12556/RUL-155351
UDC:
577
ISSN on article:
2041-1723
DOI:
10.1038/s41467-023-37765-6
COBISS.SI-ID:
150022147
Publication date in RUL:
27.03.2024
Views:
239
Downloads:
350
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Record is a part of a journal
Title:
Nature communications
Shortened title:
Nat. Commun.
Publisher:
Nature Publishing Group
ISSN:
2041-1723
COBISS.SI-ID:
2315876
Licences
License:
CC BY 4.0, Creative Commons Attribution 4.0 International
Link:
http://creativecommons.org/licenses/by/4.0/
Description:
This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
Secondary language
Language:
Slovenian
Keywords:
biokemija
,
proteini
,
beljakovine
,
antigenski receptorji
,
rakava obolenja
Projects
Funder:
ARRS - Slovenian Research Agency
Project number:
P4-0176
Name:
Molekularna biotehnologija: od dinamike bioloških sistemov do aplikacij
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