Tomato brown rugose fruit virus in aqueous environments : survival and significance of water-mediated transmission

Mehle, Nataša (author), Bačnik, Katarina (author), Bajde, Irena (author), Brodarič, Jakob (author), Fox, Adrian (author), Gutiérrez-Aguirre, Ion (author), Kitek, Miha (author), Kutnjak, Denis (author), Loh, Yue Lin (author), Maksimović Carvalho Ferreira, Olivera (author), Ravnikar, Maja (author), Vogel, Elise (author), Vos, Christine (author), Vučurović, Ana (author)

Tomato brown rugose fruit virus (ToBRFV) has recently emerged as a major disease of tomatoes and peppers. ToBRFV is a seed- and contact-transmitted virus. In Slovenia, ToBRFV RNA was detected in samples of wastewater, river, and water used to irrigate plants. Even though the source of detected RNA could not be clearly established, this raised the question of the significance of the detection of ToBRFV in water samples and experimental studies were performed to address this question. The data presented here confirm that the release of virus particles from the roots of infected plants is a source of infectious ToBRFV particles in water and that the virus can remain infective up to four weeks in water stored at room temperature, while its RNA can be detected for much longer. These data also indicate that irrigation with ToBRFV-contaminated water can lead to plant infection. In addition, it has been shown that ToBRFV circulated in drain water in commercial tomato greenhouses from other European countries and that an outbreak of ToBRFV can be detected by regular monitoring of drain water. A simple method for concentrating ToBRFV from water samples and a comparison of the sensitivity of different methods, including the determination of the highest ToBRFV dilution still capable of infecting test plants, were also investigated. The results of our studies fill the knowledge gaps in the epidemiology and diagnosis of ToBRFV, by studying the role of water-mediated transmission, and provide a reliable risk assessment to identify critical points for monitoring and control.

• Language: English
• Keywords: tomato brown rugose fruit virus, tomato, hydroponics, water-linked epidemiology, survival
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 15 str.
• Numbering: Vol. 14, art. 1187920
• PID: 20.500.12556/RUL-155269
• UDC: 632
• ISSN on article: 1664-462X
• DOI: 10.3389/fpls.2023.1187920
• COBISS.SI-ID: 154635011
• Publication date in RUL: 22.03.2024

Record is a part of a journal

• Title: Frontiers in plant science
• Shortened title: Front. plant sci.
• Publisher: Frontiers Media
• ISSN: 1664-462X
• COBISS.SI-ID: 3011663

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: virus rjave grbančavosti paradižnika, paradižnik, hidroponija, epidemiologija