Omics mass spectrometry analysis of canine plasma

Schlosser, Gitta (author), Molnár, Adrienn (author), Papp, Dávid (author), Gellén, Gabriella (author), Virág, Dávid (author), Ludányi, Krisztina (author), Dalmadi-Kiss, Borbála (author), Arko, Matevž (author), Iglič, Aleš (author), Nemec Svete, Alenka (author), Erjavec, Vladimira (author), Kralj-Iglič, Veronika (author)

Blood derived products, such as autologous plasma, have high clinical importance and are applied in numerous therapeutic fields. The preparation of autologous plasma from the patient's own blood is easy to perform by centrifugation, however, the preparation procedure can significantly affect the blood cells, platelets and vesicles in the sample. Therefore, it is of utmost importance to understand the impact of sample processing on the chemical composition of plasma preparations as well as on their biological activity. Here, we present a mass spectrometry-based plasma profiling method in which three compound groups: lipids, proteins and glycoproteins are analysed in a single workflow. Analysis of the chemical composition of plasma samples prepared by different centrifugation protocols revealed differences in the lipid and glycoprotein profiles, demonstrating the importance of standardized protocols for the preparation of plasma products.

• Language: English
• Keywords: plasma, lipids, proteins, glycoproteins, mass spectrometry, ion mobility
• Typology: 1.08 - Published Scientific Conference Contribution
• Organization: ZF - Faculty of Health Sciences; FE - Faculty of Electrical Engineering; VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: Str. 67-72
• PID: 20.500.12556/RUL-155013
• UDC: 577
• DOI: 10.55295/PSL.2024.I11
• COBISS.SI-ID: 188787459
• Publication date in RUL: 14.03.2024

Record is a part of a monograph

• Title: Socratic Lectures : 10th international symposium, Ljubljana, 9. December 2023 : peer reviewed proceedings, part I
• Editors: Veronika Kralj-Iglič, Yelena Istileulova, Anna Romolo
• Place of publishing: Ljubljana
• Publisher: University of Ljubljana Press
• Year: 2024
• ISBN: 978-961-297-266-0
• COBISS.SI-ID: 186758147

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.