Validation of interferometric light microscopy for assessment of extracellular particles in 250 samples of diluted plasma : preparing the path for future clinical practices

Berry, Maxence (avtor), Arko, Matevž (avtor), Romolo, Anna (avtor), Brložnik, Maja (avtor), Mrvar Brečko, Anita (avtor), Korenjak, Boštjan (avtor), Iglič, Aleš (avtor), Kadunc Kos, Vesna (avtor), Kruljc, Peter (avtor), Nemec Svete, Alenka (avtor), Erjavec, Vladimira (avtor), Kralj-Iglič, Veronika (avtor)

In recent years, the analysis of extracellular particles (EPs) has become instrumental in deciphering intercellular communication and disease biomarkers. Interferometric light microscopy (ILM) has emerged as a powerful label-free technique for real-time characterization of nanoscale particles. This contribution presents for the first time measurements of EPs directly in diluted plasma by ILM on populations of samples. The study involves the measured number density of EPs, the corrected number density of EPs (taking into account dilution of the sample) and the size of EPs. Analysis involves plasma of multiple species—canine, equine, and human. Most of the D$_h$ in all three species were confined to the interval between 130 nm and 200 nm. We found no statistically significant correlation between the corrected n and D$_h$ indicating good performance of the method. The correlation between the measured n and D$_h$ was statistically significant indicating hindered movement of EPs due to their higher number density. These results showed that high throughput measurement of number density and size of EPs in plasma is feasible.

• Jezik: Angleški jezik
• Ključne besede: extracellular vesicles, interferometric light microscopy, plasma analysis, nanoparticle characterization, videodrop technology, nanoparticles
• Tipologija: 1.08 - Objavljeni znanstveni prispevek na konferenci
• Organizacija: ZF - Zdravstvena fakulteta ; FE - Fakulteta za elektrotehniko; MF - Medicinska fakulteta ; VF - Veterinarska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: Str. 54-58
• PID: 20.500.12556/RUL-155004
• UDK: 577
• DOI: 10.55295/PSL.2024.I9
• COBISS.SI-ID: 188588547
• Datum objave v RUL: 13.03.2024

Gradivo je del monografije

• Naslov: Socratic Lectures : 10th international symposium, Ljubljana, 9. December 2023 : peer reviewed proceedings, part I
• Uredniki: Veronika Kralj-Iglič, Yelena Istileulova, Anna Romolo
• Kraj izida: Ljubljana
• Založnik: University of Ljubljana Press
• Leto izida: 2024
• ISBN: 978-961-297-266-0
• COBISS.SI-ID: 186758147

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.