Validation of interferometric light microscopy for assessment of extracellular particles in 250 samples of diluted plasma : preparing the path for future clinical practices

Berry, Maxence (author), Arko, Matevž (author), Romolo, Anna (author), Brložnik, Maja (author), Mrvar Brečko, Anita (author), Korenjak, Boštjan (author), Iglič, Aleš (author), Kadunc Kos, Vesna (author), Kruljc, Peter (author), Nemec Svete, Alenka (author), Erjavec, Vladimira (author), Kralj-Iglič, Veronika (author)

In recent years, the analysis of extracellular particles (EPs) has become instrumental in deciphering intercellular communication and disease biomarkers. Interferometric light microscopy (ILM) has emerged as a powerful label-free technique for real-time characterization of nanoscale particles. This contribution presents for the first time measurements of EPs directly in diluted plasma by ILM on populations of samples. The study involves the measured number density of EPs, the corrected number density of EPs (taking into account dilution of the sample) and the size of EPs. Analysis involves plasma of multiple species— canine, equine, and human. Most of the Dh in all three species were confined to the interval between 130 nm and 200 nm. We found no statistically significant correlation between the corrected n and Dh indicating good performance of the method. The correlation between the measured n and Dh was statistically significant indicating hindered movement of EPs due to their higher number density. These results showed that high throughput measurement of number density and size of EPs in plasma is feasible.

• Language: English
• Keywords: extracellular vesicles, interferometric light microscopy, plasma analysis, nanoparticle characterization, videodrop technology, nanoparticles
• Work type: Scientific work
• Typology: 1.08 - Published Scientific Conference Contribution
• Organization: ZF - Faculty of Health Sciences; FE - Faculty of Electrical Engineering; VF - Veterinary Faculty
• Year: 2024
• Number of pages: Str. 54-58
• PID: 20.500.12556/RUL-155004
• UDC: 577
• DOI: 10.55295/PSL.2024.I9
• COBISS.SI-ID: 188588547
• Publication date in RUL: 13.03.2024

Record is a part of a monograph

• Title: Socratic Lectures : 10th international symposium, Ljubljana, 9. December 2023
• Editors: Veronika Kralj-Iglič, Yelena Istileulova, Anna Romolo
• Place of publishing: Ljubljana
• Publisher: University of Ljubljana Press
• Year: 2024
• ISBN: 978-961-297-266-0
• COBISS.SI-ID: 186758147

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.