Analytics of therapeutic monoclonal antibody (mAb) is covered by the use of a wide range of analytical methods. In our work we focused on the preparation of samples with the TECAN Freedom EVO® 200 robotic system for the purpose of capillary gel electrophoresis in the presence of sodium dodecyl sulfate (CE-SDS) in reducing and non-reducing conditions. In the first phase of our work we evaluated the appropriate mode of sample injection (hydrodynamic and electrophoretic) for both CE-SDS methods. In the second phase we then assessed the effectiveness and comparability of samples prepared with the help of a robot compared to classical manual sample preparation. Finally, both CE-SDS methods were optimized by extending the shelf life of reagents in buffer trays where we confirmed stability of vials filled with SDS-MW gel (storage at room temperature) as well as vials filled with UPW (cold room storage at 2-8°C) for up to one month. Overall, we showed that the mode of injection has no significant impact on the values of the monitored parameters of both CE-SDS methods. The observed differences can be attributed to the variability of the methods themselves or they are the consequence of combining automatic and manual integration of electropherograms. Lastly, we have demonstrated that automated sample preparation is comparable to the classical manual sample preparation, thus confirming our main hypothesis.
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