A comparison of the oral microbiota in healthy dogs and dogs with oral tumors

Lisjak, Anja (author), Lopes, Bruna Correa (author), Pilla, Rachel (author), Nemec, Ana (author), Suchodolski, Jan S. (author), Tozon, Nataša (author)

The aim of this study was to further describe the oral microbiota of healthy dogs by DNA shotgun sequencing and compare those to dogs with oral tumors. Oral swabs (representative of all niches of the oral cavity) were collected from healthy dogs (n = 24) and from dogs with different oral tumors (n = 7). DNA was extracted from the swabs and shotgun metagenomic sequencing was performed. Only minor differences in microbiota composition were observed between the two groups. At the phylum level, the Bacteroidota, Proteobacteria, Actinobacteriota, Desulfobacterota and Firmicutes were most abundant in both groups. Observed Operational Taxonomic Units—OTUs (species richness) was significantly higher in the healthy patients, but there was no significant difference in the Shannon diversity index between the groups. No significant difference was found in beta diversity between the groups. The core oral microbiota consisted of 67 bacterial species that were identified in all 24 healthy dogs. Our study provides further insight into the composition of the oral microbiota of healthy dogs and in dogs with oral tumors.

• Language: English
• Keywords: microbiota, DNA, neoplasms, biopsy, dogs
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: VF - Veterinary Faculty
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: 19 str.
• Numbering: Vol. 13, iss. 23, art. 3594
• PID: 20.500.12556/RUL-154200
• UDC: 636.09:616
• ISSN on article: 2076-2615
• DOI: 10.3390/ani13233594
• COBISS.SI-ID: 173949187
• Publication date in RUL: 31.01.2024

Record is a part of a journal

• Title: Animals
• Shortened title: Animals
• Publisher: MDPI AG
• ISSN: 2076-2615
• COBISS.SI-ID: 519120409

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.
• Licensing start date: 31.01.2024