Microfluidic electrochemical glucose biosensor with in situ enzyme immobilization

Lokar, Nina (avtor), Pečar, Borut (avtor), Možek, Matej (avtor), Vrtačnik, Danilo (avtor)

The development and characterization of a microfluidic electrochemical glucose biosensor are presented herein. The transducer part is based on thin-film metal electrodes on a glass substrate. The biological recognition element of the biosensor is the pyrroloquinoline quinone–glucose dehydrogenase (PQQ-GdhB) enzyme, selectively in situ immobilized via microcontact printing of a mixed self-assembling monolayer (SAM) on a gold working electrode, while the microfluidic part of the device comprises microchannel and microfluidic connections formed in a polydimethylsiloxane (PDMS) elastomer. The electrode properties throughout all steps of biosensor construction and the biosensor response to glucose concentration and analyte flow rate were characterized by cyclic voltammetry and chronoamperometry. A measurement range of up to 10 mM in glucose concentration with a linear range up to 200 µM was determined. A detection limit of 30 µM in glucose concentration was obtained. Respective biosensor sensitivities of 0.79 nA/µM/mm$^2$ and 0.61 nA/µM/mm$^2$ were estimated with and without a flow at 20 µL/min. The developed approach of in situ enzyme immobilization can find a wide number of applications in the development of microfluidic biosensors, offering a path towards continuous and time-independent detection.

• Jezik: Angleški jezik
• Ključne besede: microfluidic glucose biosensor, electrochemical biosensor, thin film electrode, enzyme in situ immobilization, chronoamperometry, cyclic voltammetry
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: FE - Fakulteta za elektrotehniko
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2023
• Št. strani: 16 str.
• Številčenje: Vol. 13, iss. 3, art. 364
• PID: 20.500.12556/RUL-154088
• UDK: 602.1:53.082.9
• ISSN pri članku: 2079-6374
• DOI: 10.3390/bios13030364
• COBISS.SI-ID: 145284355
• Datum objave v RUL: 24.01.2024

Gradivo je del revije

• Naslov: Biosensors
• Skrajšan naslov: Biosensors
• Založnik: MDPI
• ISSN: 2079-6374
• COBISS.SI-ID: 523007769

Licence

• Licenca: CC BY 4.0, Creative Commons Priznanje avtorstva 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by/4.0/deed.sl
• Opis: To je standardna licenca Creative Commons, ki daje uporabnikom največ možnosti za nadaljnjo uporabo dela, pri čemer morajo navesti avtorja.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: mikrofluidni biosenzor glukoze, elektrokemični biosenzor, tankoplastna elektroda, imobilizacija encima in situ, kronoamperometrija, ciklična voltametrija

Projekti

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: P2-0244
• Naslov: Mikrostrukture in nanostrukture

• Financer: ARRS - Agencija za raziskovalno dejavnost Republike Slovenije
• Številka projekta: 1000-22-0510