Dynamics of cell death due to electroporation using different pulse parameters as revealed by diferent viability assays

Peng, Wencheng (author), Polajžer, Tamara (author), Yao, Chenguo (author), Miklavčič, Damijan (author)

The mechanisms of cell death due to electroporation are still not well understood. Recent studies suggest that cell death due to electroporation is not an immediate all-or-nothing response but rather a dynamic process that occurs over a prolonged period of time. To investigate whether the dynamics of cell death depends on the pulse parameters or cell lines, we exposed different cell lines to different pulses [monopolar millisecond, microsecond, nanosecond, and high-frequency bipolar (HFIRE)] and then assessed viability at different times using different viability assays. The dynamics of cell death was observed by changes in metabolic activity and membrane integrity. In addition, regardless of pulse or cell line, the dynamics of cell death was observed only at high electroporation intensities, i.e., high pulse amplitudes and/or pulse number. Considering the dynamics of cell death, the clonogenic assay should remain the preferred viability assay for assessing viability after electroporation.

• Language: English
• Keywords: electroporation, pulsed field ablation, cell death dynamics, pulse parameters, viability assays
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: FE - Faculty of Electrical Engineering
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: Str. 22-35
• Numbering: Vol. 52, iss. 1
• PID: 20.500.12556/RUL-154040
• UDC: 602.621
• ISSN on article: 0090-6964
• DOI: 10.1007/s10439-023-03309-8
• COBISS.SI-ID: 164795907
• Publication date in RUL: 22.01.2024

Record is a part of a journal

• Title: Annals of biomedical engineering
• Shortened title: Ann. biomed. eng.
• Publisher: Springer Nature, Biomedical Engineering Society
• ISSN: 0090-6964
• COBISS.SI-ID: 560916

Licences

• License: CC BY 4.0, Creative Commons Attribution 4.0 International
• Link: http://creativecommons.org/licenses/by/4.0/
• Description: This is the standard Creative Commons license that gives others maximum freedom to do what they want with the work as long as they credit the author.

Secondary language

• Language: Slovenian
• Keywords: elektroporacija, ablacija s pulznim električnim poljem, dinamika celične smrti, parametri pulza, testi preživetja