Antibacterial evaluation of different prosthetic liner textiles coated by CuO nanoparticles

Najmi, Ziba (author), Matijaković Mlinarić, Nives (author), Scalia, Alessandro Calogero (author), Cochis, Andrea (author), Selmani, Atiđa (author), Učakar, Aleksander (author), Abram, Anže (author), Zore, Anamarija (author), Delač Marion, Ida (author), Jerman, Ivan (author), Van de Velde, Nigel Willy (author), Vidmar, Janja (author), Bohinc, Klemen (author), Rimondini, Lia (author)

Prosthetic liners are mainly used as an interface between residual limbs and prosthetic sockets to minimize physical and biological damage to soft tissue. However, the closed and moist conditions within liners and the amputee's skin provide a suitable environment for bacterial growth to cause infections. This study aimed to coat a comprehensive variant material with copper oxide nanoparticles (CuO NPs) and compare their surface analysis and antibacterial properties. These materials were covered with CuO NPs solution at a concentration of 70 μg mL−1 to achieve this purpose. After drying, their surface characteristics were analyzed by measuring zeta potential, contact angle, surface roughness, and fiber arrangement. Cu-released concentration from the coatings into the acetate buffer solution by inductively coupled plasma mass spectrometry indicated that lycra and nylon quickly released Cu ions to concentrations up to ∼0.2 μg mL−1 after 24 h, causing low metabolic activity of human bone–marrow mesenchymal stem cells (bMSC) in the indirect assay. Antibacterial activity of the coated specimens was evaluated by infecting their surfaces with the Gram-positive bacteria Staphylococcus epidermidis, reporting a significant ∼40 % reduction of metabolic activity for x-dry after 24 h; in addition, the number of viable bacterial colonies adhered to the surface of this material was reduced by ∼23 times in comparison with non-treated x-dry that were visually confirmed by scanning electron microscope. In conclusion, CuO NPs x-dry shows optimistic results to pursue further experiments due to its slow speed of Cu release and prolonged antibacterial activity, as well as its compatibility with human cells.

• Language: English
• Keywords: prosthetic liner, textile, copper, nanoparticle, antibacterial action, cytocompatibility
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: ZF - Faculty of Health Sciences
• Publication status: Published
• Publication version: Version of Record
• Publication date: 01.01.2024
• Year: 2024
• Number of pages: 11 str.
• Numbering: Vol. 10, issue 1, art. e23849
• PID: 20.500.12556/RUL-153464
• UDC: 620.3:544
• ISSN on article: 2405-8440
• DOI: 10.1016/j.heliyon.2023.e23849
• COBISS.SI-ID: 178063363
• Publication date in RUL: 08.01.2024

Record is a part of a journal

• Title: Heliyon
• Publisher: Elsevier
• ISSN: 2405-8440
• COBISS.SI-ID: 21607432

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: protetična podloga, tekstil, baker, nanodelci, antibakterijsko delovanje, citokompatibilnost

Projects

• Funder: ARIS - Slovenian Research and Innovation Agency
• Project number: N1-0264-2022
• Name: Antibakterijske in protivirusne lastnosti nano prevlečenih površin