Analiza proteinske korone izbranih nanodelcev, izpostavljenih humanemu serumu in vitro.

Furlan Kovač, Manca (author), Pavlin, Mojca (mentor), Leonardi, Adrijana (comentor)

Proteinska korona, ki nastane okoli nanodelcev v fizioloških medijih, ima pomembno vlogo pri biokompatibilnosti nanodelcev. Cilj naše raziskave je bil določanje sestave proteinske korone izbranih nanodelcev izpostavljenih krvnemu serumu zdravih posameznikov. Nanodelce smo inkubirali s humanim serumom, da se je formirala proteinska korona. Proteine korone smo ločili na NaDS-PAGE elektroforeznem gelu in jih identificirali s pomočjo masne spektrometrije. Ugotovili smo, kateri izmed nanodelcev nase vežejo največ proteinov in kako se sestava proteinske korone med njimi razlikuje. Z identifikacijo proteinov, ki se vežejo na izbrane nanodelce v krvnem serumu, smo poglobili razumevanje možnih vplivov različnih nanodelcev na fiziološko ravnovesje po vstopu v organizem. Zanimala nas je sestava proteinske korone, razlike med različnimi nanodelci ter specifično interakcije s proteini, ki bi potencialno lahko povzročili morebitne neželene učinke nanodelcev na procese, kot so strjevanje krvi in aktivacija komplementa ter trombocitov. Potrdili smo hipotezo, da se sestava proteinske korone razlikuje med izbranimi nanodelci ter predvideli vpletenost identificiranih proteinov v procesih hemostaze in imunskega odziva. Glede na rezultate naše raziskave lahko sklepamo, da so izbrani nanodelci vpleteni v nepričakovane interakcije s proteini krvi, kar bi potencialno lahko vplivalo na uspešnost biomedicinskih postopkov, pri katerih se uporabljajo nanodelci. Prav tako bi lahko omenjene interakcije vodile do nezaželenih imunskih odzivov in drugih učinkov. Posledično bi lahko nanodelci predstavljali dejavnik tveganja za zdravje človeka zaradi vse večje izpostavljenosti nanodelcem.

• Language: Slovenian
• Keywords: biotehnologija, nanodelci, proteinska korona, nanoAg, nanoTiO2, nanoCoFe2O4
• Work type: Master's thesis/paper
• Typology: 2.09 - Master's Thesis
• Organization: BF - Biotechnical Faculty
• Year: 2023
• PID: 20.500.12556/RUL-153352
• COBISS.SI-ID: 179031043
• Publication date in RUL: 23.12.2023

FURLAN KOVAČ, Manca, 2023, Analiza proteinske korone izbranih nanodelcev, izpostavljenih humanemu serumu in vitro. [online]. Master’s thesis. [Accessed 14 April 2025]. Retrieved from: https://repozitorij.uni-lj.si/IzpisGradiva.php?lang=eng&id=153352

Secondary language

• Language: English
• Title: Protein corona analysis of selected nanoparticles, exposed to human serum in vitro.

Abstract

Protein corona that forms around nanoparticles in physiological media plays an important role in the biocompatibility of nanoparticles. The aim of our research was to determine the composition of the protein corona of selected nanoparticles in the blood serum of healthy individuals. We incubated nanoparticles with human serum to form the protein corona. We separated the corona proteins on a SDS-PAGE electrophoresis gel and identified them by mass spectrometry. We determined which nanoparticles bind the highest number of proteins and how the composition of the protein corona differs between nanoparticles. By identifying the proteins that bind to selected nanoparticles in blood serum, we deepened our understanding of the potential effects of different nanoparticles on physiological balance after their entry into the body. We were interested in the composition of the protein corona, the differences between different nanoparticles, and in the specific interactions with proteins that could potentially cause adverse effects of nanoparticles on processes such as blood coagulation, complement and platelet activation. We have confirmed the hypothesis that the composition of the protein corona differs between the selected nanoparticles and demonstrated the involvement of the identified proteins in the processes of hemostasis and immune response. Based on the results of our research, we can conclude that the selected nanoparticles are involved in unexpected interactions with blood proteins, which could potentially impact the success of biomedical procedures involving nanoparticles. Additionally, these interactions could lead to undesirable immune responses and other effects. Consequently, nanoparticles could represent a risk factor for human health due to increasing exposure to nanoparticles.

• Keywords: biotechnology, nanoparticles, protein corona, nanoAg, nanoTiO2, nanoCoFe2O4