Infectious diseases are one of the main causes of death and are spreading successfully in today's age due to the way of living. Their consequences have an impact on many systems. Mesenchymal stem/stromal cells are becoming more and more attractive in the treatment of viral diseases due to their self-renewal, differentiation, anti-inflammatory and immunomodulatory properties. The properties of these cells mostly depend on the donor and tissue source. In this thesis, we aimed to determine the properties of bone marrow-derived MSCs of a young healthy donor such astheir proliferation, differentiation and immunomodulatory potential in a sample of manually handled cells or cells grown in a Quantum bioreactor. To determine the proliferation capacity, the cells were grown in vitro until the cells started to die off. To determine the differentiation capacity we performed adipogenesis, osteogenesis and chondrogenesis of MSCs in vitro. To test the immunomodulatory properties of MSC, we applied conditioned media to Jurkat cell culture. We observed that the cells, regardless of the method of previous cultivation in vitro begin to die in the tenth passage. In the trilineage differentiation test, the adipogenic differentiation proved to be the most successful as shown by both, histology and the expression of the adipogenic genes. In the immunomodulatory test, we did not observe differences in the effect on proliferation between individual samples. Our results suggest that these bone marrow cells have potential for applications in viral diseases due to their MSC-like properties. Further tests on stimulated immune system cells are needed to determine their immunomodulatory properties.
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