The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets

Goršak, Tanja (avtor), Jarc Jovičić, Eva (avtor), Tratnjek, Larisa (avtor), Križaj, Igor (avtor), Sepulveda, Borja (avtor), Nogues, Josep (avtor), Erdani-Kreft, Mateja (avtor), Petan, Toni (avtor), Kralj, Slavko (avtor), Makovec, Darko (avtor)

Magneto-mechanical actuation (MMA) using the low-frequency alternating magnetic fields (AMFs) of magnetic nanoparticles internalized into cancer cells can be used to irreparably damage these cells. However, nanoparticles in cells usually agglomerate, thus greatly augmenting the delivered force compared to single nanoparticles. Here, we demonstrate that MMA also decreases the cell viability, with the MMA mediated by individual, non-interacting nanoparticles. The effect was demonstrated with ferrimagnetic (i.e., permanently magnetic) barium-hexaferrite nanoplatelets (NPLs, ~50 nm wide and 3 nm thick) with a unique, perpendicular orientation of the magnetization. Two cancer-cell lines (MDA-MB-231 and HeLa) are exposed to the NPLs in-vitro under different cell-culture conditions and actuated with a uniaxial AMF. TEM analyses show that only a small number of NPLs internalize in the cells, always situated in membrane-enclosed compartments of the endosomal-lysosomal system. Most compartments contain 1–2 NPLs and only seldom are the NPLs found in small groups, but never in close contact or mutually oriented. Even at low concentrations, the single NPLs reduce the cell viability when actuated with AMFs, which is further increased when the cells are in starvation conditions. These results pave the way for more efficient in-vivo MMA at very low particle concentrations.

• Jezik: Angleški jezik
• Ključne besede: cancer cells, magneto-mechanical actuation, hexaferrite nanoplatelets, cancer, cell viability, colloidal stability
• Vrsta gradiva: Članek v reviji
• Tipologija: 1.01 - Izvirni znanstveni članek
• Organizacija: MF - Medicinska fakulteta
• Status publikacije: Objavljeno
• Različica publikacije: Objavljena publikacija
• Leto izida: 2024
• Št. strani: Str. 778-787
• Številčenje: Vol. 657
• PID: 20.500.12556/RUL-153015
• UDK: 577:537
• ISSN pri članku: 1095-7103
• DOI: 10.1016/j.jcis.2023.12.019
• COBISS.SI-ID: 176772867
• Datum objave v RUL: 14.12.2023

Gradivo je del revije

• Naslov: Journal of colloid and interface science
• Skrajšan naslov: J. colloid interface sci.
• Založnik: Elsevier
• ISSN: 1095-7103
• COBISS.SI-ID: 527041561

Licence

• Licenca: CC BY-NC-ND 4.0, Creative Commons Priznanje avtorstva-Nekomercialno-Brez predelav 4.0 Mednarodna
• Povezava: http://creativecommons.org/licenses/by-nc-nd/4.0/deed.sl
• Opis: Najbolj omejujoča licenca Creative Commons. Uporabniki lahko prenesejo in delijo delo v nekomercialne namene in ga ne smejo uporabiti za nobene druge namene.

Sekundarni jezik

• Jezik: Slovenski jezik
• Ključne besede: rakotvorne celice