The efficient magneto-mechanical actuation of cancer cells using a very low concentration of non-interacting ferrimagnetic hexaferrite nanoplatelets

Goršak, Tanja (author), Jarc Jovičić, Eva (author), Tratnjek, Larisa (author), Križaj, Igor (author), Sepulveda, Borja (author), Nogues, Josep (author), Erdani-Kreft, Mateja (author), Petan, Toni (author), Kralj, Slavko (author), Makovec, Darko (author)

Magneto-mechanical actuation (MMA) using the low-frequency alternating magnetic fields (AMFs) of magnetic nanoparticles internalized into cancer cells can be used to irreparably damage these cells. However, nanoparticles in cells usually agglomerate, thus greatly augmenting the delivered force compared to single nanoparticles. Here, we demonstrate that MMA also decreases the cell viability, with the MMA mediated by individual, non-interacting nanoparticles. The effect was demonstrated with ferrimagnetic (i.e., permanently magnetic) barium-hexaferrite nanoplatelets (NPLs, ~50 nm wide and 3 nm thick) with a unique, perpendicular orientation of the magnetization. Two cancer-cell lines (MDA-MB-231 and HeLa) are exposed to the NPLs in-vitro under different cell-culture conditions and actuated with a uniaxial AMF. TEM analyses show that only a small number of NPLs internalize in the cells, always situated in membrane-enclosed compartments of the endosomal-lysosomal system. Most compartments contain 1–2 NPLs and only seldom are the NPLs found in small groups, but never in close contact or mutually oriented. Even at low concentrations, the single NPLs reduce the cell viability when actuated with AMFs, which is further increased when the cells are in starvation conditions. These results pave the way for more efficient in-vivo MMA at very low particle concentrations.

• Language: English
• Keywords: cancer cells, magneto-mechanical actuation, hexaferrite nanoplatelets, cancer, cell viability, colloidal stability
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: MF - Faculty of Medicine
• Publication status: Published
• Publication version: Version of Record
• Year: 2024
• Number of pages: Str. 778-787
• Numbering: Vol. 657
• PID: 20.500.12556/RUL-153015
• UDC: 577:537
• ISSN on article: 1095-7103
• DOI: 10.1016/j.jcis.2023.12.019
• COBISS.SI-ID: 176772867
• Publication date in RUL: 14.12.2023

Record is a part of a journal

• Title: Journal of colloid and interface science
• Shortened title: J. colloid interface sci.
• Publisher: Elsevier
• ISSN: 1095-7103
• COBISS.SI-ID: 527041561

Licences

• License: CC BY-NC-ND 4.0, Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International
• Link: http://creativecommons.org/licenses/by-nc-nd/4.0/
• Description: The most restrictive Creative Commons license. This only allows people to download and share the work for no commercial gain and for no other purposes.

Secondary language

• Language: Slovenian
• Keywords: rakotvorne celice