Surfactin molecules with a cone-like structure promote the formation of membrane domains with negative spontaneous curvature and induce membrane invaginations

Pandur, Žiga (author), Penič, Samo (author), Iglič, Aleš (author), Kralj-Iglič, Veronika (author), Stopar, David (author), Drab, Mitja (author)

Surfactin uniquely influences lipid bilayer structure by initially inducing membrane invaginations before solubilization. In this study, we exposed DOPC giant vesicles to various surfactin concentrations at different temperatures and observed surfactin-induced membrane invaginations by using differential interference contrast and confocal laser fluorescence microscopy. These invaginations were stable at room temperature but not at higher temperatures. Surfactin molecules induce membrane nanodomains with negative spontaneous curvature and membrane invaginations despite their intrinsic conical shape and intrinsic positive curvature. Considering the experimentally observed capacity of surfactin to fluidize lipid acyl chains and induce partial dehydration of lipid headgroups, we propose that the resulting surfactin-lipid complexes exhibit a net negative spontaneous curvature. We further conducted 3D numerical Monte Carlo (MC) simulations to investigate the behaviour of vesicles containing negative curvature nanodomains within their membrane at varying temperatures. MC simulations demonstrated strong agreement with experimental results, revealing that invaginations are preferentially formed at low temperatures, while being less pronounced at elevated temperatures. Our findings go beyond the expectations of the Israelachvili molecular shape and packing concepts analysis. These concepts do not take into account the influence of specific interactions between neighboring molecules on the inherent shapes of molecules and their arrangement within curved membrane nanodomains. Our work contributes to a more comprehensive understanding of the complex factors governing vesicle morphology and membrane organization and provides insight into the role of detergent-lipid interactions in modulating vesicle morphology.

• Language: English
• Keywords: surfactin, lipid membranes, solubilization dynamics, curved membrane nanodomains, molecular shape
• Work type: Article
• Typology: 1.01 - Original Scientific Article
• Organization: BF - Biotechnical Faculty; FE - Faculty of Electrical Engineering; MF - Faculty of Medicine; ZF - Faculty of Health Sciences
• Publication status: Published
• Publication version: Version of Record
• Year: 2023
• Number of pages: Str. 1193-1200
• Numbering: Vol. 650, pt. B
• PID: 20.500.12556/RUL-153008
• UDC: 577.352
• ISSN on article: 0021-9797
• DOI: 10.1016/j.jcis.2023.07.057
• COBISS.SI-ID: 159013379
• Publication date in RUL: 14.12.2023

Record is a part of a journal

• Title: Journal of colloid and interface science
• Shortened title: J. colloid interface sci.
• Publisher: Elsevier
• ISSN: 0021-9797
• COBISS.SI-ID: 5255941

Licences

• License: CC BY-NC 4.0, Creative Commons Attribution-NonCommercial 4.0 International
• Link: http://creativecommons.org/licenses/by-nc/4.0/
• Description: A creative commons license that bans commercial use, but the users don’t have to license their derivative works on the same terms.

Secondary language

• Language: Slovenian
• Keywords: lipidne membrane, surfaktin