Vaš brskalnik ne omogoča JavaScript!
JavaScript je nujen za pravilno delovanje teh spletnih strani. Omogočite JavaScript ali pa uporabite sodobnejši brskalnik.
Nacionalni portal odprte znanosti
Odprta znanost
DiKUL
slv
|
eng
Iskanje
Brskanje
Novo v RUL
Kaj je RUL
V številkah
Pomoč
Prijava
DNA modifications impact natural transformation of Acinetobacter baumannii
ID
Vesel, Nina
(
Avtor
),
ID
Iseli, Christian
(
Avtor
),
ID
Guex, Nicolas
(
Avtor
),
ID
Lemopoulos, Alexandre
(
Avtor
),
ID
Blokesch, Melanie
(
Avtor
)
PDF - Predstavitvena datoteka,
prenos
(1,69 MB)
MD5: 7508B9A82359DC6E565644387A9EFB81
URL - Izvorni URL, za dostop obiščite
https://academic.oup.com/nar/article/51/11/5661/7161540
Galerija slik
Izvleček
Acinetobacter baumannii is a dangerous nosocomial pathogen, especially due to its ability to rapidly acquire new genetic traits, including antibiotic resistance genes (ARG). In A. baumannii, natural competence for transformation, one of the primary modes of horizontal gene transfer (HGT), is thought to contribute to ARG acquisition and has therefore been intensively studied. However, knowledge regarding the potential role of epigenetic DNA modification(s) on this process remains lacking. Here, we demonstrate that the methylome pattern of diverse A. baumannii strains differs substantially and that these epigenetic marks influence the fate of transforming DNA. Specifically, we describe a methylome-dependent phenomenon that impacts intra- and inter-species DNA exchange by the competent A. baumannii strain A118. We go on to identify and characterize an A118-specific restriction-modification (RM) system that impairs transformation when the incoming DNA lacks a specific methylation signature. Collectively, our work contributes towards a more holistic understanding of HGT in this organism and may also aid future endeavors towards tackling the spread of novel ARGs. In particular, our results suggest that DNA exchanges between bacteria that share similar epigenomes are favored and could therefore guide future research into identifying the reservoir(s) of dangerous genetic traits for this multi-drug resistant pathogen.
Jezik:
Angleški jezik
Ključne besede:
Acinetobacter baumannii
,
transformation
,
restriction-modification system
Vrsta gradiva:
Članek v reviji
Tipologija:
1.01 - Izvirni znanstveni članek
Organizacija:
BF - Biotehniška fakulteta
Status publikacije:
Objavljeno
Različica publikacije:
Objavljena publikacija
Leto izida:
2023
Št. strani:
Str. 5661–5677
Številčenje:
Vol. 51, iss. 11
PID:
20.500.12556/RUL-152988
UDK:
577
ISSN pri članku:
1362-4962
DOI:
10.1093/nar/gkad377
COBISS.SI-ID:
177332995
Datum objave v RUL:
13.12.2023
Število ogledov:
396
Število prenosov:
11
Metapodatki:
Citiraj gradivo
Navadno besedilo
BibTeX
EndNote XML
EndNote/Refer
RIS
ABNT
ACM Ref
AMA
APA
Chicago 17th Author-Date
Harvard
IEEE
ISO 690
MLA
Vancouver
:
Kopiraj citat
Objavi na:
Gradivo je del revije
Naslov:
Nucleic acids research online
Skrajšan naslov:
Nucleic acids res. online
Založnik:
Oxford University Press
ISSN:
1362-4962
COBISS.SI-ID:
2172954
Podobna dela
Podobna dela v RUL:
Podobna dela v drugih slovenskih zbirkah:
Nazaj